Supplied as 0.22um filtered solution in PBS (pH 7.4).
The product should be stored at -70℃. Please do not repeated freeze-thaw cycles.
Biotinylated Recombinant human EGFR VIII Protein, His tag on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Recombinant human EGFR VIII Protein is greater than 95% as determined by SEC-HPLC.
Immobilized Biotinylated human EGFR VIII,His Tag at 0.2ug/ml (100ul/Well). Dose response curve for Anti-EGFRvIII mAb with EC50 of 27.99ng/ml determined by ELISA.
The epidermal growth factor receptor (EGFR) is overexpressed in a variety of human epithelial tumors, often as a consequence of gene amplification. Tumors with EGFR gene amplification frequently contain EGFR gene rearrangements, with the most common extracellular domain mutation being EGFRvIII. This mutation leads to a deletion of exons 2-7 of the EGFR gene and renders the mutant receptor incapable of binding any known ligand.