CD73 antigen; CD73; NT5E; NTE; 5' nucleotidase (CD73); 5'-NT; 5-NT; 5'-Nucleotidase; 5'-nucleotidase, ecto (CD73); E5NT; EC 18.104.22.168; ecto-5'-nucleotidase; eN; eNT; NT; NT55'-nucleotidase;Purine 5-Prime-Nucleotidase
Supplied as 0.22um filtered solution in 20mM Tris-HCl, 120mM NaCl,4mM CaCl2, 20% Glycerol, pH 7.5.
The product should be stored at -70℃. Please do not repeated freeze-thaw cycles.
Recombinant CD73 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
The purity of CD73 (Cat. CD7-HM173) is greater than 95% as determined by SEC-HPLC.
Measured by its ability to hydrolyze the 5’-phosphate group from the substrate adenosine-5’-monophosphate (AMP). The orthophosphate product is measured by a Malachite Green Phosphate Detection Kit . The specific activity is >15,000 pmol/min/μg.
Immobilized Human CD73 at 0.5ug/ml(100ul/Well). Dose response curve for Anti-CD73 Ab. with the EC50 of 16.9ng/ml determined by ELISA.
CD73, also known as ecto-5'-nucleotidase, is an enzyme that in humans is encoded by the NT5E gene.CD73 commonly serves to convert AMP to adenosine.The enzyme consists of a dimer of 2 identical 70-kD subunits bound by a glycosyl phosphatidyl inositol linkage to the external face of the plasma membrane. The enzyme is used as a marker of lymphocyte differentiation. A deficiency of CD73 occurs in a variety of immunodeficiency diseases.