SLA-HM448B | 价格

SLA-HM448B-100μg / 询价

SLA-HM448B-500μg / 询价

SLA-HM448B-500μgx2 / 询价

Biotinylated Human CD48/SLAMF2 Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Biotinylated Human CD48/SLAMF2 Protein is expressed from HEK293 with His tag and Avi Tag at the C-terminus.
It contains Gln27-Ser220 [Accession | P09326-1].

分子量大小(Molecular Weight)

The protein has a predicted MW of 25.25 kDa. Due to glycosylation, the protein migrates to 45-60 kDa based on Bis-Tris PAGE result.

纯度(Purity)

> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC

内毒素(Endotoxin)

Less than 1 EU per μg by the LAL method.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Bis-Tris PAGE
Biotinylated Human CD48 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
SEC-HPLC
The purity of Biotinylated Human CD48 is greater than 95% as determined by SEC-HPLC.
ELISA Data
Immobilized Biotinylated Human CD48, His-Avi Tag at 2μg/ml(100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Human 2B4, hFc Tag with the EC50 of 0.9ng/ml determined by ELISA. (QC Test)
背景(Background)

CD48, also known as BLAST-1, BCM-1, and SLAMF2, is a 65 kDa GPI-linked protein in the CD2 family of immunoglobulin superfamily molecules.CD2 and 2B4 (CD244) are known ligands for CD48. CD48 protein is expressed on most lineage-committed hematopoietic cells but not on hematopoietic stem cells or multipotent hematopoietic progenitors.

分子别名(Synonyms)

CD48 molecule; CD48; SLAMF2; BCM1; BLAST; BLAST1; BLAST1TCT.1;  mCD48; MEM-102; TCT.1

文献(References)

(1)Velikovsky C A , Deng L , Chlewicki L K , et al. Structure of Natural Killer Receptor 2B4 Bound to CD48 Reveals Basis for Heterophilic Recognition in Signaling Lymphocyte Activation Molecule Family[J]. Immunity, 2007, 27(4):0-584.

Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.