货号价格

2B4-HM101-100μg / 询价

2B4-HM101-500μg / 询价

2B4-HM101-500μgx2 / 询价

Human 2B4/CD244/SLAMF4 Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Human 2B4/CD244/SLAMF4 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Cys22-Arg221.[Accession | Q9BZW8-2]

分子量大小(Molecular Weight)

The protein has a predicted MW of 23.1 kDa. Due to glycosylation, the protein migrates to 45-70 kDa based on Tris-Bis PAGE result.

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

纯度(Purity)

> 95% as determined by Tris-Bis PAGE
> 95% as determined by HPLC

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3-6 months after reconstitution.
2-8°C for 2-7 days after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Tris-Bis PAGE

Human 2B4 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Human 2B4 is greater than 95% as determined by SEC-HPLC.

ELISA Data

Immobilized Human 2B4, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human CD48, hFc Tag with the EC50 of 0.34μg/ml determined by ELISA.

背景(Background)

2B4 (CD244) is expressed by memory-phenotype CD8 T cells and all natural killer (NK) cells. The ligand for 2B4, CD48, is expressed on hematopoietic cells. 2B4 is conserved in humans and mice, and a number of reports have linked 2B4 with activation of lymphocytes.Engagement of 2B4 on NK cell surfaces with specific antibodies or CD48 can trigger cell mediated cytotoxicity, interferon γ secretion, phosphoinositol turnover and NK cell invasiveness.

分子别名(Synonyms)

2B4; CD244 antigen; CD244; h2B4; NAIL; NKR2B4; Nmrk; SLAMF4

文献(References)

(1)Velikovsky C A , Deng L , Chlewicki L K , et al. Structure of Natural Killer Receptor 2B4 Bound to CD48 Reveals Basis for Heterophilic Recognition in Signaling Lymphocyte Activation Molecule Family[J]. Immunity, 2007, 27(4):0-584.

Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lypholized powder.