Nanodisc系列蛋白

Nanodisc也称纳米圆盘，为一种由磷脂和膜支架分子组成的类膜结构，是用于研究膜蛋白结构和功能的重要工具。它通过模拟天然磷脂双分子层膜环境，为膜蛋白提供了一个稳定的平台，利用Nanodisc展示多次跨膜蛋白不仅可以更好地维持蛋白的天然构象，还可解决多次跨膜蛋白的可溶性制备问题，广泛应用于药物开发的各种分析检测过程。

定制服务

Nanodisc系列蛋白 — Fig. 1 Nanodisc展示多次跨膜蛋白示意图

恺佧生物基于独特的 SMA（Styrene-Maleic Anhydride）Nanodisc平台，采用无去垢剂工艺，直接从细胞膜上原位提取目标蛋白，在保留内源性脂质微环境的同时，将目标蛋白包裹为均一、可溶的纳米颗粒，最大程度保留了膜蛋白的天然构象与生物学活性。

SMA共聚物从细胞膜提取多次跨膜蛋白并纯化形成Nanodisc的制备过程 — Fig. 2 Nanodisc制备示意图

产品特点

天然构象：完整保留了膜蛋白的天然结构与正确构象
功能确认：确保蛋白具备真实的生物学活性
标签可选：提供His-tag和生物素化标签可选

应用方向

抗体筛选
分子水平功能表征
检测方法开发：ELISA、SPR、BLI等

数据示例

恺佧生物开发的Nanodisc展示全长GPRC5D蛋白具有优异的生物学活性和较高的检测灵敏度。

Human GPRC5D Nanodisc与抗体结合的剂量响应曲线 — Fig. 3 Immobilized Human GPRC5D Nanodisc, His Tag at 2 μg/ml (100 μl/well) on the plate. Dose response curve for Anti-GPRC5D Antibody, hFc Tag with the EC₅₀ of 4.9 ng/ml determined by ELISA.

Human GPRC5D Nanodisc与抗体结合的SPR检测曲线 — Fig. 4 Human GPRC5D Nanodisc, His Tag captured on CM5 Chip via anti-his antibody can bind Anti-GPRC5D Antibody with an affinity constant of 1.47 nM as determined in SPR assay.

为更大程度助益抗体药物（如双抗药物）开发，恺佧生物特别提供生物素化的Nanodisc展示多次跨膜蛋白，在测评中表现出优异的结合活性，可灵活应用于ELISA方法开发及药代动力学分析等不同研究阶段。

生物素化GPRC5D Nanodisc用于双特异性抗体的ELISA检测曲线 — Fig. 5 Immobilized Human CD3E&CD3D, His Tag at 2 μg/ml (100 μl/well) on the plate, add serial dilutions of Anti-Human CD3×GPRC5D Bispecific Antibody, hFc Tag and then add Biotinylated Human GPRC5D Nanodisc, His Tag at 5 μg/ml. Detection was performed using HRP-conjugated streptavidin with the EC₅₀ of 0.28 μg/ml determined by ELISA.

生物素化GPRC5D Nanodisc与抗体结合的BLI检测曲线 — Fig. 6 Loaded Biotinylated Human GPRC5D Nanodisc, His Tag on SA-Biosensor, can bind Anti-GPRC5D Antibody with an affinity constant of 1.16 nM as determined in BLI assay.

产品列表

蛋白名称	货号	产品名称	标签	表达系统
A2AR	A2R-HM1N1	Human A2AR Nanodisc	C-His	HEK293
CCR4	CCR-HM14NB	Biotinylated Human CCR4 Nanodisc	C-His	HEK293
CCR4	CR4-HM1N122	Human CCR4 Nanodisc	C-His	HEK293
CCR6	CCR-HM16NB	Biotinylated Human CCR6 Nanodisc	C-His	HEK293
CCR7	CCR-HM17NB	Biotinylated Human CCR7 Nanodisc	C-His	HEK293
CCR7	CCR-HM107	Human CCR7 Nanodisc	C-His	HEK293
CCR8	CR8-HM1N29	Human CCR8 Nanodisc	C-His	HEK293
CCR9	CCR-HM19NB	Biotinylated Human CCR9 Nanodisc	C-His	HEK293
CD3	TCC-HM101	Biotinylated Human NY-ESO-1 TCR&CD3 Complex Nanodisc	C-His	HEK293
CD3	TCC-HM102	Biotinylated Human KRAS G12D TCR&CD3 Complex Nanodisc	C-His	HEK293
CD20	CD2-DM12NB	Biotinylated Canine CD20/MS4A1 Nanodisc	C-His	HEK293
CD20	CD2-HM12NB	Biotinylated Human CD20/MS4A1 Nanodisc	C-His	HEK293
CD81	CD8-HM18NB	Biotinylated Human CD81 Nanodisc	C-His	HEK293
CD133	CD1-HM13NB	Biotinylated Human CD133 Nanodisc	C-His	HEK293
Claudin 18.2	CLD-HM10NB	Biotinylated Human Claudin 18.2 Nanodisc	C-His	HEK293
Claudin 1	CLD-HM1N1B	Biotinylated Human Claudin 1 Nanodisc	C-His	HEK293
Claudin 3	CLD-HM13NB	Biotinylated Human Claudin 3 Nanodisc	C-His	HEK293
Claudin 4	CLD-HM14NB	Biotinylated Human Claudin 4 Nanodisc	C-His	HEK293
Claudin 4	CLD4-HM1N161	Human Claudin 4 Nanodisc	C-His	HEK293
Claudin 6	CLD-HM16NB	Biotinylated Human Claudin 6 Nanodisc	C-His	HEK293
CRTh2	CRT-HM1HNB	Biotinylated Human CRTh2 Nanodisc	C-His	HEK293
CX3CR1	CXR-HM10NB	Biotinylated Human CX3CR1 Nanodisc	C-His	HEK293
CXCR5	CXC-HM1RNB	Biotinylated Human CXCR5 Nanodisc	C-His	HEK293
CXCR5	CXR5-HM1N72	Human CXCR5 Nanodisc	C-His	HEK293
CXCR6	CXC-HM16NB	Biotinylated Human CXCR6 Nanodisc	C-His	HEK293
CXCR7	CXR-HM17NB	Biotinylated Human CXCR7 Nanodisc	C-His	HEK293
EDNRA	EDA-HM10VB	Biotinylated Human EDNRA Nanodisc	C-His	HEK293
GCGR	GCR-HM10NB	Biotinylated Human GCGR/Glucagon receptor Nanodisc	C-His	HEK293
GIPR	GIP-HM14RNB	Biotinylated Human GIPR Nanodisc	C-His	HEK293
GLP-1R	GLP-HM10NB	Biotinylated Human GLP-1R Nanodisc	C-His	HEK293
GLP-1R	GLP-HM1N123	Human GLP-1R Nanodisc	C-His	HEK293
GPRC5D	GPR-HM15NB	Biotinylated Human GPRC5D Nanodisc	C-His	HEK293
GPRC5D	GPR-HM15P	Human GPRC5D Nanodisc	C-His	HEK293
Kv1.3	KV1-HM10NB	Biotinylated Human Kv1.3 Nanodisc	C-His	HEK293
LGR-4	LGR-HM10N	Human LGR-4 Nanodisc	C-His	HEK293
LPAR1	LAR-HM10NB	Biotinylated Human LPAR1 Nanodisc	C-His	HEK293
MRGPRX2	MR2-HM10XN	Biotinylated Human MRGPRX2 Nanodisc	C-His	HEK293
MRGPRX2	MR2-HM1N118	Human MRGPRX2 Nanodisc	C-His	HEK293
NaPi2b	SLC-HM1ANB	Biotinylated Human NaPi2b/SLC34A2 Nanodisc	C-His	HEK293
PC2	PC2-HM10N	Human PC2/Polycystin-2 Nanodisc	C-His	HEK293
SLC15A4	SLC-HM15NB	Biotinylated Human SLC15A4 Nanodisc	C-His	HEK293
SLC6A17	SLC-HM17NB	Biotinylated Human SLC6A17 Nanodisc	C-His	HEK293
SSTR2	STR-HM10N	Biotinylated Human SSTR2 Nanodisc	C-His	HEK293
SSTR2	STR-HM4N159B	Biotinylated Human SSTR2 Nanodisc	C-His-Avi	HEK293
TM4SF1	TSF-HM00N	Human TM4SF1 Nanodisc	N-His	HEK293
Nanodisc Control	CON-HM1NB	Biotinylated Nanodisc Control	C-His	HEK293