恺佧生物科技（上海）有限公司

表达区间及表达系统（Source）	Recombinant Biotinylated Human CD133 Nanodisc is expressed from HEK293 with His tag at the C-terminus. It contains Met1-His865 [Accession \| O43490-1].
分子量大小（Molecular Weight）	The protein has a predicted MW of 110.1 kDa.
内毒素（Endotoxin）	Less than 1EU per μg by the LAL method.
制剂（Formulation）	Supplied as 0.22 μm filtered solution in PBS, 200mM L-arginine (pH 7.4). Notice: Not recommended for flow cytometry in mammalian cells.
存储（Storage）	Valid for 6 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
阴性对照（Recommended Negative Control）	CON-HM1NB

Schematic diagram of Nanodisc preparation

Our Styrene-Maleic Anhydride(SMA) nanodisc platform delivers multi-pass transmembrane proteins exactly as they exist in the HEK293 cell membrane following recombinant expression— preserving their endogenous lipid environment and correct conformational states. Isolated directly from HEK293 cells via a gentle, detergent-free process, the multi-pass membrane proteins are subsequently encapsulated by SMA to ensure their stability. These uniform soluble nanoparticles eliminate extraction interferences while maintaining biological activity. Ensuring consistent performance from lot to lot, our nanodiscs provide the reliability and biological relevance essential for antibody screening and functional characterization in therapeutic antibody drug discovery and development, as assessed by SPR, BLI, ELISA, and other standard platforms.

Features

❖  Correct structure & conformation
❖  Functional relevance ensured
❖  His-tag & biotinylation tag available

Applications

❖  Antibody screening
❖  Functional characterization(Molecular Level)
❖  Assay: SPR, BLI, ELISA

表达区间及表达系统（Source）

Recombinant Biotinylated Human CD133 Nanodisc is expressed from HEK293 with His tag at the C-terminus.
It contains Met1-His865 [Accession | O43490-1].

分子量大小（Molecular Weight）

The protein has a predicted MW of 110.1 kDa.

内毒素（Endotoxin）

Less than 1EU per μg by the LAL method.

制剂（Formulation）

Supplied as 0.22 μm filtered solution in PBS, 200mM L-arginine (pH 7.4). Notice: Not recommended for flow cytometry in mammalian cells.

存储（Storage）

Valid for 6 months from date of receipt when stored at -80°C.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

阴性对照（Recommended Negative Control）

CON-HM1NB