KACTUS-CAS9-1 mg / 询价
KACTUS-CAS9-3 mg / 询价
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Cas9 Nuclease
Cas9 核酸酶克隆自野生型酿脓链球菌 Streptococcus pyogenes，可实现对造血干细胞、T 细胞等的基因修饰。本品经密码子优化、核定位信号(NLS)的设计、大肠杆菌表达体系与纯化工艺的优化等步骤生产得来。
|
表达系统（Source） |
E.coli;Asp2-Asp1368; [Accession | Q99ZW2] |
|
种属（Species） |
Streptococcus pyogenes |
|
分子量大小（Molecular Weight） |
The protein has a predicted MW of 163 kDa. |
|
浓度（Concentration） |
10 mg/ml |
|
纯度（Purity）(Bis-Tris PAGE) |
≥95% |
|
纯度（Purity）(SEC-HPLC) |
≥95% |
|
内毒素（Endotoxin） |
≤10 EU/mg |
|
储存缓冲液（Storage Buffer） |
30 mM Tris-HCl, 300 mM NaCl, 0.1 mM EDTA, 50% Glycerol, pH 7.4 |
|
运输/保存条件（Transportation/Storage Condition） |
干冰运输，-20 ± 5℃保存，避免反复冻融 |
|
产品应用（Product Applications） |
参与细胞与基因治疗药物（造血干细胞、T细胞等）的基因修饰。 |
The purity of Cas9 Nuclease detected by Bis-Tris PAGE is greater than 95%.
The purity of Cas9 Nuclease detected by SEC-HPLC is greater than 95%.
The in vitro cleavage activity of the three batches of Cas9 Nuclease was basically the same.
Three batches of Kactus Cas9 Nuclease were electrotransfected into 293T cell line to knockout the target gene. As shown, the knockout efficiency in the three batches were basically comparable.
