KACTUS-CAS9-1 mg / 询价
KACTUS-CAS9-3 mg / 询价
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Cas9 Nuclease
Cas9 核酸酶克隆自野生型酿脓链球菌 Streptococcus pyogenes，可实现对造血干细胞、T 细胞等的基因修饰，也可以应用于分子诊断领域，实现对病原体的高灵敏度、高特异性检测。本品经密码子优化、核定位信号(NLS)的设计、大肠杆菌表达体系与纯化工艺的优化等步骤生产得来。
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表达区间与表达系统（Source） |
E.coli;Asp2-Asp1368; [Accession | Q99ZW2] |
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种属（Species） |
Streptococcus pyogenes |
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纯度（Purity）(SEC-HPLC) |
≥95% |
|
纯度（Purity）(RP-HPLC) |
≥95% |
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浓度（Concentration） |
10 mg/ml |
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储存缓冲液（Storage Buffer） |
30 mM Tris-HCl, 300 mM NaCl, 0.1 mM EDTA, 50% Glycerol, pH 7.4 |
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内毒素（Endotoxin） |
≤10 EU/mg |
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运输/保存条件（Transportation/Storage Condition） |
干冰运输，-20 ± 5℃保存，避免反复冻融 |
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产品应用（Product Applications） |
参与细胞与基因治疗药物（造血干细胞、T细胞等）的基因修饰。 |
Fig. 1 The purity of Cas9 Nuclease detected by Bis-Tris PAGE is greater than 95%.
Fig. 2 The purity of Cas9 Nuclease detected by SEC-HPLC is greater than 95%.
Fig. 3 The in vitro cleavage activity of the three batches of Cas9 Nuclease was basically the same.
Fig. 4 Three batches of Kactus Cas9 Nuclease were electrotransfected into 293T cell line to knockout the target gene. As shown, the knockout efficiency in the three batches were basically comparable.
