货号价格

KACTUS-CAS9-1 mg / 询价

KACTUS-CAS9-3 mg / 询价

Cas9 Nuclease

COA DS
COA DS
产品描述(Product Description)

Cas9 核酸酶克隆自野生型酿脓链球菌 Streptococcus pyogenes,可实现对造血干细胞、T 细胞等的基因修饰,也可以应用于分子诊断领域,实现对病原体的高灵敏度、高特异性检测。本品经密码子优化、核定位信号(NLS)的设计、大肠杆菌表达体系与纯化工艺的优化等步骤生产得来。

产品信息 (Product Info)

表达区间与表达系统(Source)

E.coli;Asp2-Asp1368; [Accession | Q99ZW2]

种属(Species)

Streptococcus pyogenes

纯度(Purity)(SEC-HPLC)

≥95%

纯度(Purity)(RP-HPLC)

≥95%

浓度(Concentration)

10 mg/ml

储存缓冲液(Storage Buffer)

30 mM Tris-HCl, 300 mM NaCl, 0.1 mM EDTA, 50% Glycerol, pH 7.4

内毒素(Endotoxin)

≤10 EU/mg

运输/保存条件(Transportation/Storage Condition)

干冰运输,-20 ± 5℃保存,避免反复冻融

产品应用(Product Applications)

参与细胞与基因治疗药物(造血干细胞、T细胞等)的基因修饰。

产品数据 (Assay Data)
Bis-Tris PAGE

Fig. 1 The purity of Cas9 Nuclease detected by Bis-Tris PAGE is greater than 95%.

SEC-HPLC

Fig. 2 The purity of Cas9 Nuclease detected by SEC-HPLC is greater than 95%.

Three batches of in vitro cleavage activity

Fig. 3 The in vitro cleavage activity of the three batches of Cas9 Nuclease was basically the same.

Three batches of gene knockout efficiency

Fig. 4 Three batches of Kactus Cas9 Nuclease were electrotransfected into 293T cell line to knockout the target gene. As shown, the knockout efficiency in the three batches were basically comparable.