CXC-HM16NB | 价格

CXC-HM16NB-100μg / 询价

CXC-HM16NB-100μgx5 / 询价

CXC-HM16NB-100μgx10 / 询价

Biotinylated Human CXCR6 Nanodisc

产品信息(Product Info)
Schematic diagram of Nanodisc preparation

Our Styrene-Maleic Anhydride(SMA) nanodisc platform delivers multi-pass transmembrane proteins exactly as they exist in the HEK293 cell membrane following recombinant expression— preserving their endogenous lipid environment and correct conformational states. Isolated directly from HEK293 cells via a gentle, detergent-free process, the multi-pass membrane proteins are subsequently encapsulated by SMA to ensure their stability. These uniform soluble nanoparticles eliminate extraction interferences while maintaining biological activity. Ensuring consistent performance from lot to lot, our nanodiscs provide the reliability and biological relevance essential for antibody screening and functional characterization in therapeutic antibody drug discovery and development, as assessed by SPR, BLI, ELISA, and other standard platforms.

Features

❖  Correct structure & conformation
❖  Functional relevance ensured
❖  His-tag & biotinylation tag available

Applications

❖  Antibody screening
❖  Functional characterization(Molecular Level)
❖  Assay: SPR, BLI, ELISA

表达区间及表达系统(Source)

Recombinant Biotinylated Human CXCR6 Nanodisc is expressed from HEK293 with His tag at the C-terminus.
It contains Met1-Leu342 [Accession | O00574].

分子量大小(Molecular Weight)

The protein has a predicted MW of 52.2 kDa.

内毒素(Endotoxin)

Less than 1 EU per μg by the LAL method.

制剂(Formulation)

Supplied as 0.22 μm filtered solution in PBS, 200mM L-arginine (pH 7.4). Notice: Not recommended for flow cytometry in mammalian cells.

存储(Storage)

Valid for 6 months from date of receipt when stored at -80°C.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

阴性对照(Recommended Negative Control)
CON-HM1NB
产品数据(Assay Data)
ELISA Data
Immobilized Biotinylated Human CXCR6 Nanodisc, His Tag at 1μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-CXCR6 Antibody, hFc Tag with the EC50 of 3.2ng/ml determined by ELISA. (QC Test)
SPR Data
Biotinylated Human CXCR6 Nanodisc, His Tag captured on CM5 Chip via Streptavidin can bind Anti-CXCR6 Antibody, hFc Tag with an affinity constant of 5.96 pM as determined in SPR assay (Biacore S200).
背景(Background)

CXCR6 is a receptor for the chemokine CXCL16, which exists as a membrane or soluble form. CXCR6 is a marker for resident memory T (TRM) cells that plays a role in immunosurveillance through their interaction with epithelial cells. The interaction of CXCR6 with CXCL16 expressed at the membrane of certain subpopulations of intratumor dendritic cells (DC) called DC3, ideally positions these CXCR6+ T cells to receive a proliferation signal from IL-15 also presented by DC3.

分子别名(Synonyms)

CXC-R6; CXCR-6; CDw186; CD186; BONZO; STRL33; TYMSTR;CXCR6

文献(References)

Mabrouk N, Tran T, Sam I, Pourmir I, Gruel N, Granier C, Pineau J, Gey A, Kobold S, Fabre E, Tartour E. CXCR6 expressing T cells: Functions and role in the control of tumors. Front Immunol. 2022 Oct 12;13:1022136. doi: 10.3389/fimmu.2022.1022136. PMID: 36311728; PMCID: PMC9597613.