CXR5-HM1N72-100μg / 询价
CXR5-HM1N72-100μg x 5 / 询价
CXR5-HM1N72-100μg x 10 / 询价
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FITC-equivalent Human CXCR5 Protein-Nanodisc
Recombinant FITC-equivalent Human CXCR5 Protein-Nanodisc is expressed from HEK293 with His tag at the C-terminus.It contains Met1-Phe372 [Accession | P32302-1].
The protein has a predicted MW of 43.3 kDa.
Excitation Wavelength: 490 nm
Emission Wavelength: 520 nm
Less than 1EU per μg by the LAL method.
Supplied as 0.22 μm filtered solution in PBS (pH 7.4). Notice: Not recommended for immunization.
Valid for 12 months from date of receipt when stored at -80°C.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized FITC-equivalent Human CXCR5, His Tag at 5μg/ml(100μl/well) on the plate. Dose response curve for Anti-CXCR5 Antibody, hFc Tag with the EC50 of 11.7ng/ml determined by ELISA (QC Test).
CXCR5 is a serpentine receptor implicated in cell migration in lymphocytes and differentiation in leukocytes. It causes MAPK pathway activation and has known membrane partners for signaling. CXCR5 is also expressed in HL-60 cells, a human acute myeloid leukemia line, following treatment with all-trans retinoic acid, which induces differentiation toward a neutrophil-like state. CXCR5 is necessary for this process; differentiation was crippled in CXCR5 knockout cells and enhanced in cells ectopically expressing it.
CXC-R5; CXCR-5; MDR-15; MDR15; CD185; BLR1
MacDonald RJ, Yen A. CXCR5 overexpression in HL-60 cells enhances chemotaxis toward CXCL13 without anticipated interaction partners or enhanced MAPK signaling. In Vitro Cell Dev Biol Anim. 2018 Dec;54(10):725-735. doi: 10.1007/s11626-018-0293-z. Epub 2018 Oct 1. Erratum in: In Vitro Cell Dev Biol Anim. 2020 Oct;56(9):825. doi: 10.1007/s11626-020-00510-0. PMID: 30276608; PMCID: PMC6657802.
