LAR-HM10NB | 价格

LAR-HM10NB-100μg / 询价

LAR-HM10NB-100μgx5 / 询价

LAR-HM10NB-100μgx10 / 询价

Biotinylated Human LPAR1 Nanodisc

产品信息(Product Info)
Schematic diagram of Nanodisc preparation

Our Styrene-Maleic Anhydride(SMA) nanodisc platform delivers multi-pass transmembrane proteins exactly as they exist in the HEK293 cell membrane following recombinant expression— preserving their endogenous lipid environment and correct conformational states. Isolated directly from HEK293 cells via a gentle, detergent-free process, the multi-pass membrane proteins are subsequently encapsulated by SMA to ensure their stability. These uniform soluble nanoparticles eliminate extraction interferences while maintaining biological activity. Ensuring consistent performance from lot to lot, our nanodiscs provide the reliability and biological relevance essential for antibody screening and functional characterization in therapeutic antibody drug discovery and development, as assessed by SPR, BLI, ELISA, and other standard platforms.

Features

❖  Correct structure & conformation
❖  Functional relevance ensured
❖  His-tag & biotinylation tag available

Applications

❖  Antibody screening
❖  Functional characterization(Molecular Level)
❖  Assay: SPR, BLI, ELISA

表达区间及表达系统(Source)

Recombinant Biotinylated Human LPAR1 Nanodisc is expressed from HEK293 with His tag at the C-terminus.
It contains Met1-Val364 [Accession | Q92633-1].

分子量大小(Molecular Weight)

The protein has a predicted MW of 54.10 kDa.

内毒素(Endotoxin)

Less than 1 EU per μg by the LAL method.

制剂(Formulation)

Supplied as 0.22 μm filtered solution in PBS, 200mM L-arginine (pH 7.4). Notice: Not recommended for flow cytometry in mammalian cells.

存储(Storage)

Valid for 6 months from date of receipt when stored at -80°C.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

阴性对照(Recommended Negative Control)
CON-HM1NB
产品数据(Assay Data)
ELISA Data
Immobilized Biotinylated Human LPAR1 Nanodisc, His Tag at 5μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-LPAR1 Antibody, hFc Tag with the EC50 of 17.6ng/ml determined by ELISA. (QC Test)
SPR Data
Biotinylated Human LPAR1 Nanodisc, His Tag captured on CM5 Chip via Streptavidin can bind Anti-LPAR1 Antibody, hFc Tag with an affinity constant of 0.13 μM as determined in SPR assay (Biacore T200).
背景(Background)

Lysophosphatidic acid receptor 1 (LPA1) belongs to the G protein-coupled receptor family. The ligand for LPA1 is LPA, the simplest lysophospholipid. The mature form of LPA1 is glycosylated and localized on the plasma membrane. LPA1 is bound to heterotrimetric G proteins and transduces intracellular signaling in response to ligation to LPA. Desensitization of LPA1 negatively regulates LPA1-mediated signaling and the resulting biological functions.

分子别名(Synonyms)

LPA receptor 1; LPA-1; EDG2; LPA1; Gpcr26

文献(References)

Zhao J, Stephens T, Zhao Y. Molecular Regulation of Lysophosphatidic Acid Receptor 1 Maturation and Desensitization. Cell Biochem Biophys. 2021 Sep;79(3):477-483. doi: 10.1007/s12013-021-00999-6. Epub 2021 May 25. PMID: 34032994; PMCID: PMC8887818.