CCR-HM107 | 价格

CCR-HM107-100μg / 询价

CCR-HM107-100μgx5 / 询价

CCR-HM107-100μgx10 / 询价

Human CCR7 Nanodisc

产品信息(Product Info)
Schematic diagram of Nanodisc preparation

Our Styrene-Maleic Anhydride(SMA) nanodisc platform delivers multi-pass transmembrane proteins exactly as they exist in the HEK293 cell membrane following recombinant expression— preserving their endogenous lipid environment and correct conformational states. Isolated directly from HEK293 cells via a gentle, detergent-free process, the multi-pass membrane proteins are subsequently encapsulated by SMA to ensure their stability. These uniform soluble nanoparticles eliminate extraction interferences while maintaining biological activity. Ensuring consistent performance from lot to lot, our nanodiscs provide the reliability and biological relevance essential for antibody screening and functional characterization in therapeutic antibody drug discovery and development, as assessed by SPR, BLI, ELISA, and other standard platforms.

Features

❖  Correct structure & conformation
❖  Functional relevance ensured
❖  His-tag & biotinylation tag available

Applications

❖  Antibody screening
❖  Functional characterization(Molecular Level)
❖  Assay: SPR, BLI, ELISA

表达区间及表达系统(Source)

Recombinant Human CCR7 Nanodisc is expressed from HEK293 with His tag at the C-terminus (FITC-equivalent protein is fused on cytoplasmic part).
It contains Met1-Pro378 [Accession | P32248].

分子量大小(Molecular Weight)

The protein has a predicted MW of 67.70 kDa.

内毒素(Endotoxin)

Less than 1 EU per μg by the LAL method.

制剂(Formulation)

Supplied as 0.22 μm filtered solution in PBS, 200mM L-arginine (pH 7.4). Notice: Not recommended for flow cytometry in mammalian cells.

存储(Storage)

Valid for 6 months from date of receipt when stored at -80°C.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

阴性对照(Recommended Negative Control)
CON-HM1NB
产品数据(Assay Data)
ELISA Data
Immobilized Human CCR7 Nanodisc, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Anti-CCR7 Antibody, hFc Tag with the EC50 of 49.2ng/ml determined by ELISA. (QC Test)
SPR Data
Human CCR7 Nanodisc, His Tag captured on CM5 Chip via Anti-his antibody can bind Anti-CCR7 Antibody, hFc Tag with an affinity constant of 4.11 nM as determined in SPR assay (Biacore T200).
背景(Background)

CC-chemokine receptor 7 (CCR7), collaborated with its ligands CCL19 and CCL21, controls extensive migratory events in the immune system. CCR7-bearing dendritic cells can swarm into T-cell zones in lymph nodes, initiating the antigen presentation and T-cell response. Abnormal expression of CCR7 in dendritic cells will cause a series of inflammatory diseases due to the chaotic dendritic cell trafficking.

分子别名(Synonyms)

CCR7; C-C CKR-7; CC-CKR-7; CCR-7; BLR2; CDw197; EBI1; CD197; CMKBR7; EBI1; EVI1;C-C CKR7; CC-CKR7

文献(References)

Hong W, Yang B, He Q, Wang J, Weng Q. New Insights of CCR7 Signaling in Dendritic Cell Migration and Inflammatory Diseases. Front Pharmacol. 2022 Feb 25;13:841687. doi: 10.3389/fphar.2022.841687. PMID: 35281921; PMCID: PMC8914285.