SLC-HM1ANB | 价格

SLC-HM1ANB-100μg / 询价

SLC-HM1ANB-100μgx5 / 询价

SLC-HM1ANB-100μgx10 / 询价

Biotinylated Human NaPi2b/SLC34A2 Nanodisc

产品信息(Product Info)
Schematic diagram of Nanodisc preparation

Our Styrene-Maleic Anhydride(SMA) nanodisc platform delivers multi-pass transmembrane proteins exactly as they exist in the HEK293 cell membrane following recombinant expression— preserving their endogenous lipid environment and correct conformational states. Isolated directly from HEK293 cells via a gentle, detergent-free process, the multi-pass membrane proteins are subsequently encapsulated by SMA to ensure their stability. These uniform soluble nanoparticles eliminate extraction interferences while maintaining biological activity. Ensuring consistent performance from lot to lot, our nanodiscs provide the reliability and biological relevance essential for antibody screening and functional characterization in therapeutic antibody drug discovery and development, as assessed by SPR, BLI, ELISA, and other standard platforms.

Features

❖  Correct structure & conformation
❖  Functional relevance ensured
❖  His-tag & biotinylation tag available

Applications

❖  Antibody screening
❖  Functional characterization(Molecular Level)
❖  Assay: SPR, BLI, ELISA

表达区间及表达系统(Source)

Recombinant Biotinylated Human NaPi2b/SLC34A2 Nanodisc is expressed from HEK293 with His tag at the C-terminus.
It contains Met1-Leu689 [Accession | O95436-2].

分子量大小(Molecular Weight)

The protein has a predicted MW of 88.6 kDa.

内毒素(Endotoxin)

Less than 1 EU per μg by the LAL method.

制剂(Formulation)

Supplied as 0.22 μm filtered solution in PBS, 200mM L-arginine (pH 7.4). Notice: Not recommended for flow cytometry in mammalian cells.

存储(Storage)

Valid for 6 months from date of receipt when stored at -80°C.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

阴性对照(Recommended Negative Control)
CON-HM1NB
产品数据(Assay Data)
ELISA Data
Immobilized Biotinylated Human NaPi2b Nanodisc, His Tag at 2μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Lifastuzumab, hFc Tag with the EC50 of 10.8ng/ml determined by ELISA. (QC Test)
 
Immobilized Biotinylated Human NaPi2b Nanodisc, His Tag at 2μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-SLC34A2 Antibody 2, hFc Tag with the EC50 of 4.5ng/ml determined by ELISA.
SPR Data
Biotinylated Human NaPi2b Nanodisc, His Tag captured on CM5 Chip via Streptavidin can bind Lifastuzumab, hFc Tag with an affinity constant of 2.64 nM as determined in SPR assay (Biacore T200).
背景(Background)

SLC34A2, a member of the SLC34 family, is a multipass membrane protein and encodes a type 2b sodium-dependent phosphate transporter, NaPiIIb. It is known that SLC34A2 can mediate transporting inorganic phosphate into epithelial cells via sodium ion cotransport. SLC34A2 also strongly inhibited tumor growth and metastasis ability in an A549 subcutaneous tumor model and lung metastasis mode.

分子别名(Synonyms)

NaPi-2b; NaPi 2b; NAPI-IIb; NPTIIb; NaPi2b; PULAM;

文献(References)

Li Y, Chen X, Lu H. Knockdown of SLC34A2 Inhibits Hepatocellular Carcinoma Cell Proliferation and Invasion. Oncol Res. 2016 Oct 27;24(6):511-519. doi: 10.3727/096504016X14719078133483. PMID: 28281971; PMCID: PMC7838622.