GIP-HM14RNB | 价格

GIP-HM14RNB-100μg / 询价

GIP-HM14RNB-100μgx5 / 询价

GIP-HM14RNB-100μgx10 / 询价

Biotinylated Human GIPR Nanodisc

产品信息(Product Info)
Schematic diagram of Nanodisc preparation

Our Styrene-Maleic Anhydride(SMA) nanodisc platform delivers multi-pass transmembrane proteins exactly as they exist in the HEK293 cell membrane following recombinant expression— preserving their endogenous lipid environment and correct conformational states. Isolated directly from HEK293 cells via a gentle, detergent-free process, the multi-pass membrane proteins are subsequently encapsulated by SMA to ensure their stability. These uniform soluble nanoparticles eliminate extraction interferences while maintaining biological activity. Ensuring consistent performance from lot to lot, our nanodiscs provide the reliability and biological relevance essential for antibody screening and functional characterization in therapeutic antibody drug discovery and development, as assessed by SPR, BLI, ELISA, and other standard platforms.

Features

❖  Correct structure & conformation
❖  Functional relevance ensured
❖  His-tag & biotinylation tag available

Applications

❖  Antibody screening
❖  Functional characterization(Molecular Level)
❖  Assay: SPR, BLI, ELISA

表达区间及表达系统(Source)

Recombinant Biotinylated Human GIPR Nanodisc is expressed from HEK293 with His tag at the C-terminus.
It contains Met1-Cys466 [Accession | P48546-1].

分子量大小(Molecular Weight)

The protein has a predicted MW of 63.8 kDa.

内毒素(Endotoxin)

Less than 1 EU per μg by the LAL method.

制剂(Formulation)

Supplied as 0.22 μm filtered solution in PBS, 200mM L-arginine (pH 7.4). Notice: Not recommended for flow cytometry in mammalian cells.

存储(Storage)

Valid for 6 months from date of receipt when stored at -80°C.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

阴性对照(Recommended Negative Control)
CON-HM1NB
产品数据(Assay Data)
ELISA Data
Immobilized Biotinylated Human GIPR Nanodisc, His Tag at 5μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-GIPR Antibody, hFc Tag with the EC50 of 25.5ng/ml determined by ELISA (QC Test). (QC Test)
SPR Data
Biotinylated Human GIPR Nanodisc, His Tag captured on CM5 Chip via Streptavidin can bind Anti-GIPR Antibody, hFc Tag with an affinity constant of 18.70 nM as determined in SPR assay (Biacore T200).
BLI Data
Loaded Biotinylated Human GIPR Nanodisc, His Tag on Streptavidin-Biosensor can bind Anti-GIPR Antibody, hFc Tag with an affinity constant of 19.60 nM as determined in BLI assay .
背景(Background)

The gastric inhibitory polypeptide receptor (GIPR), a G protein-coupled receptor (GPCR) that regulates glucose metabolism and insulin secretion, is a target for the development of therapeutic agents to address type 2 diabetes and obesity.

分子别名(Synonyms)

GIP-R; GIPR; PGQTL2

文献(References)

Brown KA, Morris RK, Eckhardt SJ, Ge Y, Gellman SH. Phosphorylation Sites of the Gastric Inhibitory Polypeptide Receptor (GIPR) Revealed by Trapped-Ion-Mobility Spectrometry Coupled to Time-of-Flight Mass Spectrometry (TIMS-TOF MS). J Am Chem Soc. 2023 Dec 27;145(51):28030-28037. doi: 10.1021/jacs.3c09078. Epub 2023 Dec 13. PMID: 38091482; PMCID: PMC10842860.