Biotinylated Human ANGPTL3/Angiopoietin-like 3 Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Biotinylated Human ANGPTL3/Angiopoietin-like 3 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Ser17-Lys219.[Accession | Q9Y5C1]

分子量大小(Molecular Weight)

The protein has a predicted MW of 26.6 kDa. Due to glycosylation, the protein migrates to 30-38 kDa based on Tris-Bis PAGE result.

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

纯度(Purity)

> 95% as determined by Tris-Bis PAGE
> 95% as determined by HPLC

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3-6 months after reconstitution.
2-8°C for 2-7 days after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Tris-Bis PAGE

Biotinylated Human ANGPTL3 on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%.

SEC-HPLC

The purity of Biotinylated Human ANGPTL3 is greater than 95% as determined by SEC-HPLC.

ELISA Data

Immobilized Anti-ANGPTL3 Antibody, hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human ANGPTL3, His Tag with the EC50 of 10.2ng/ml determined by ELISA.

背景(Background)

ANGPTL3 is a secreted glycoprotein that is structurally related to the angiopoietins. Mature human ANGPTL3 contains an N-terminal coiled coil domain and a C‑terminal fibrinogen-like domain. ANGPTL3 is expressed in the liver from early in development through adulthood. Acts in part as a hepatokine that is involved in regulation of lipid and glucose metabolism. Proposed to play a role in the trafficking of energy substrates to either storage or oxidative tissues in response to food intake.

分子别名(Synonyms)

ANGPTL3; ANGPT5; ANG-5; Angiopoietin-5; FHBL2; ANL3; Angiopoietin-5; Angiopoietin-like Protein 3

文献(References)

(1)Oike Y , Akao M , Kubota Y , et al. Angiopoietin-like proteins: potential new targets for metabolic syndrome therapy[J]. Trends in Molecular Medicine, 2005, 11(10):473-479.D

Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lypholized powder.