APR-MM113 | 价格

APR-MM113-100μg / 询价

APR-MM113-500μg / 询价

APR-MM113-500μgx2 / 询价

Mouse APRIL/TNFSF13 Protein (active trimer)

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Mouse APRIL/TNFSF13 Protein is expressed from HEK293 with His tag and Flag tag at the N-Terminus.
It contains Ala96-Leu241 [Accession | Q9D777].

分子量大小(Molecular Weight)

The protein has a predicted MW of 18.53 kDa. Due to glycosylation, the protein migrates to 24-30 kDa based on Bis-Tris PAGE result.

纯度(Purity)

> 90% as determined by Bis-Tris PAGE
> 90% as determined by HPLC

内毒素(Endotoxin)

Less than 1 EU per μg by the LAL method.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, 8% trehalose (pH 7.4).

重构方法(Reconstitution)

Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Bis-Tris PAGE
Mouse APRIL on Bis-Tris PAGE under reduced condition. The purity is greater than 90%.
SEC-HPLC
The purity of Mouse APRIL is greater than 90% as determined by SEC-HPLC.
SEC-MALS
The purity of Mouse APRIL is greater than 90% and the molecular weight of this protein is around 68-90 kDa as determined by SEC-MALS.
ELISA Data
Immobilized Mouse APRIL, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Mouse BCMA, hFc Tag with the EC50 of 2.2ng/ml determined by ELISA.
BLI Data
Loaded Mouse APRIL, His Tag on Anti-His-Biosensor can bind Human TACI, hFc Tag with an affinity constant of 1.98 nM as determined in BLI assay.
背景(Background)

The APRIL (a proliferation-inducing ligand), also known as TNFSF13, TALL2, TRDL1, and CD256, is a member of the TNF ligand superfamily.Both APRIL and its close relative BAFF bind and signal through the TNF superfamily receptors TACI and BCMA, while BAFF additionally functions through BAFF R.

分子别名(Synonyms)

CD256; TALL2; TALL-2; TNFSF13; APRIL; APRILFLJ57090; TRDL1; TRDL-1; ZTNF2

文献(References)

(1)Bossen C , Schneider P. BAFF, APRIL and their receptors: Structure, function and signaling[J]. Seminars in Immunology, 2006, 18(5):0-275.

Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.