BAF-CM412 | 价格

BAF-CM412-100μg / 询价

BAF-CM412-500μg / 询价

BAF-CM412-500μgx2 / 询价

Non-biotinylated Cynomolgus BAFF/TNFSF13B/CD257 Trimer Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Cynomolgus BAFF / TNFSF13B / CD257 Trimer Protein is expressed from HEK293 with His tag and Flag tag and Avi tag at the N-Terminus.
It contains Thr141-Leu285 trimer design [Accession | A0A2K5V2X4].

分子量大小(Molecular Weight)

The protein has a predicted MW of 54.2 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Bis-Tris PAGE result.

纯度(Purity)

> 90% as determined by Bis-Tris PAGE
> 85% as determined by HPLC

内毒素(Endotoxin)

Less than 1 EU per μg by the LAL method.

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in 25mM MES, 150mM NaCl, 8% trehalose (pH 6.0).

重构方法(Reconstitution)

Dissolve the lyophilized protein in 25mM MES (pH 6.0). Please refer to the Certificate of Analysis for detailed instructions.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Bis-Tris PAGE
Cynomolgus BAFF Trimer on Bis-Tris PAGE under reduced condition. The purity is greater than 90%.
SEC-HPLC
The purity of Cynomolgus BAFF Trimer is greater than 85% as determined by SEC-HPLC.
ELISA Data
Immobilized Cynomolgus BAFF Trimer, His Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Human BAFFR, hFc Tag with the EC50 of 31.6ng/ml determined by ELISA.
 
Immobilized Cynomolgus BAFF (Trimer), His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Cynomolgus/Rhesus macaque BAFFR, hFc Tag with the EC50 of 60.0ng/ml determined by ELISA.
 
Immobilized Cynomolgus BAFF Trimer, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Cynomolgus/Rhesus macaque BCMA, hFc Tag (Cat. BCM-CM217) with the EC50 of 1.5ng/ml determined by ELISA.
 
Immobilized Cynomolgus BAFF Trimer, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-BAFF Antibody, hFc Tag with the EC50 of 9.9ng/ml determined by ELISA.
BLI Data
Loaded Cynomolgus/Rhesus macaque BAFFR, hFc Tag on ProA-Biosensor can bind Cynomolgus BAFF (Trimer), His-Avi Tag with an affinity constant of 1.23 nM as determined in BLI assay.
背景(Background)

B-cell activating factor (BAFF) also known as tumor necrosis factor ligand superfamily member 13B is a protein that in humans is encoded by the TNFSF13B gene.BAFF is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for receptors TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFF-R.

分子别名(Synonyms)

BAFF; BLyS; CD257; TNFSF13B; TNFSF20; DTL; TALL1; TALL-1delta BAFF; TALL1Delta4 BAFF; THANK; ZTNF4; TALL-1

文献(References)

(1)Bossen C , Schneider P. BAFF, APRIL and their receptors: Structure, function and signaling[J]. Seminars in Immunology, 2006, 18(5):0-275.

Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.