BAF-HM4AFB | 价格

BAF-HM4AFB-100μg / 询价

BAF-HM4AFB-500μg / 询价

BAF-HM4AFB-500μgx2 / 询价

Biotinylated Human BAFF/TNFSF13B/CD257 Protein (active trimer)

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Biotinylated Human BAFF/TNFSF13B/CD257 Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus.
It contains Ala134-Leu285 [Accession | Q9Y275-1].

分子量大小(Molecular Weight)

The protein has a predicted MW of 20.72 kDa. Due to glycosylation, the protein migrates to 22-25 kDa based on Bis-Tris PAGE result.

纯度(Purity)

> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC

内毒素(Endotoxin)

Less than 1 EU per μg by the LAL method.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Bis-Tris PAGE
Biotinylated Human BAFF on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
SEC-HPLC
The purity of Biotinylated Human BAFF is greater than 95% as determined by SEC-HPLC.
SEC-MALS
The purity of Biotinylated Human BAFF is greater than 95% and the molecular weight of this protein is around 55-70 kDa as determined by SEC-MALS.
ELISA Data
Immobilized Human BAFFR, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human BAFF, His Tag with the EC50 of 8.0ng/ml determined by ELISA (QC Test).
 
Immobilized Human BCMA, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human BAFF, His Tag with the EC50 of 10.1ng/ml determined by ELISA.
 
Immobilized Biotinylated Human BAFF, His Avi Tag at 0.5μg/ml(100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-BAFF Antibody, hFc Tag with the EC50 of 6.4ng/ml determined by ELISA.
BLI Data
Loaded Biotinylated Human BAFF, His-Avi Tag on Streptavidin-Biosensor can bind Human BCMA, His Tag with an affinity constant of 0.65 µM as determined in BLI assay .
背景(Background)

B-cell activating factor (BAFF) also known as tumor necrosis factor ligand superfamily member 13B is a protein that in humans is encoded by the TNFSF13B gene.BAFF is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for receptors TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFF-R.

分子别名(Synonyms)

BAFF; BLyS; CD257; TNFSF13B; TNFSF20; DTL; TALL1; TALL-1delta BAFF; TALL1Delta4 BAFF; THANK; ZTNF4; TALL-1

文献(References)

(1)Bossen C , Schneider P. BAFF, APRIL and their receptors: Structure, function and signaling[J]. Seminars in Immunology, 2006, 18(5):0-275.

Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.