Human 4-1BB Ligand/TNFSF9 Trimer Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Human 4-1BB Ligand/TNFSF9 Trimer Protein is expressed from HEK293 with monomeric hFc tag at the N-Terminus. It contains Arg71-Glu254.[Accession | P41273]

分子量大小(Molecular Weight)

The protein has a predicted MW of 84.3 kDa same as Tris-Bis PAGE result.

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

纯度(Purity)

> 95% as determined by Tris-Bis PAGE

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3-6 months after reconstitution.
2-8°C for 2-7 days after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Tris-Bis PAGE

Human 4-1BB Ligand (Trimer) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Human 4-1BB Ligand (Trimer) is greater than 95% as determined by SEC-HPLC.

ELISA Data

Immobilized Human 4-1BB, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Human 4-1BB Ligand Trimer, hFc Tag with the EC50 of 3.3ng/ml determined by ELISA.

背景(Background)

The 4-1BBL is the high affinity ligand of 4-1BB, also known as CD137L or TNFSF9. 4-1BB ligand (4-1BBL) is an inducible molecule present on several APC types, including B cells, macrophages and DCs.4-1BB:4-1BBL pathway seems to amplify the existing costimulatory signals, even if the engagement of 4-1BB in the presence of a strong TCR signaling can induce IL-2 production in a CD28-independent manner.

分子别名(Synonyms)

41BB Ligand; 4-1BB Ligand; 4-1BBL; CD137L; TNFSF9

文献(References)

(1)Vinay D S , Kwon B S. 4-1BB (CD137), an inducible costimulatory receptor, as a specific target for cancer therapy[J]. BMB Reports, 2014, 47(3):122-129.

Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lypholized powder.