BCM-HM217 | 价格

BCM-HM217-100μg / 询价

BCM-HM217-500μg / 询价

BCM-HM217-500μgx2 / 询价

Human BCMA/TNFRSF17 Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Human BCMA/TNFRSF17 Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.
It contains Met1-Ala54 [Accession | Q02223-1].

分子量大小(Molecular Weight)

The protein has a predicted MW of 31.4 kDa. Due to glycosylation, the protein migrates to 35-45 kDa based on Bis-Tris PAGE result.

纯度(Purity)

> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC

内毒素(Endotoxin)

Less than 1 EU per μg by the LAL method.

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Bis-Tris PAGE
Human BCMA on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
SEC-HPLC
The purity of Human BCMA is greater than 95% as determined by SEC-HPLC.
ELISA Data
Immobilized Human APRIL Trimer, His Tag (Cat. APR-HM110) at 0.1μg/ml (100μl/well) on the plate. Dose response curve for Human BCMA, hFc Tag with the EC50 of 0.9ng/ml determined by ELISA (QC Test).
 
Immobilized Human BAFF Trimer, His Tag (Cat. BAF-HM112) at 2μg/ml (100μl/well) on the plate. Dose response curve for Human BCMA, hFc Tag with the EC50 of 1.7ng/ml determined by ELISA.
 
Immobilized Human BCMA, hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti-BCMA Antibody, hFc Tag with the EC50 of 5.4ng/ml determined by ELISA.
BLI Data
Loaded Human BCMA, hFc Tag on ProA-Biosensor can bind Human APRIL (Trimer), His Tag with an affinity constant of 0.24 nM as determined in BLI assay.
背景(Background)

B-cell maturation antigen (BCMA or BCM), also known as tumor necrosis factor receptor superfamily member 17 (TNFRSF17), is a protein that in humans is encoded by the TNFRSF17 gene.TNFRSF17 is a cell surface receptor of the TNF receptor superfamily which recognizes B-cell activating factor (BAFF).

分子别名(Synonyms)

CD269; TNFRSF17; BCMA; BCM; TNFRSF13A

文献(References)

(1)Bossen C , Schneider P. BAFF, APRIL and their receptors: Structure, function and signaling[J]. Seminars in Immunology, 2006, 18(5):0-275.

Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.