BH7-HM13B | 价格

BH7-HM13B-100μg / 询价

BH7-HM13B-500μg / 询价

BH7-HM13B-500μgx2 / 询价

Human B7-H3 (4Ig) /B7-H3b Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Human B7-H3 (4Ig) /B7-H3b Protein is expressed from HEK293 with His tag at the C-terminus.
It contains Gly27-Thr461 [Accession | Q5ZPR3-1].

分子量大小(Molecular Weight)

The protein has a predicted MW of 48.25 kDa. Due to glycosylation, the protein migrates to 68-78 kDa based on Bis-Tris PAGE result.

纯度(Purity)

> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC

内毒素(Endotoxin)

Less than 0.1 EU per μg by the LAL method.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Bis-Tris PAGE
Human B7-H3 (4Ig) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
SEC-HPLC
The purity of Human B7-H3 (4Ig) is greater than 95% as determined by SEC-HPLC.
ELISA Data
Immobilized Human B7-H3 (4Ig), His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-B7-H3 Antibody, hFc Tag with the EC50 of 5.0ng/ml determined by ELISA. (QC Test)
背景(Background)

B7-H3, a member of the B7 family of immunomodulatory molecules, is overexpressed in a wide range of solid cancers.B7-H3 binds to activated T cells via an as yet unidentified receptor. In assays using sub-optimal amount so anti-CD3 stimulation, 2Ig‑B7‑H3 enhances T cell proliferation, T cell interferon-gamma (IFN-gamma) production, and cytotoxic T cells induction.

分子别名(Synonyms)

B7-H3; CD276; PSEC0249; 4Ig-B7-H3; UNQ309; PRO352; B7 homolog 3; CD276

文献(References)

(1)Fodstad O , Tekle C , Chen Y W. The Immunoregulatory Protein Human B7H3 is a Tumor-Associated Antigen that Regulates Tumor Cell Migration and Invasion[J]. Current Cancer Drug Targets, 2008, 8(5)

Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.