BH7-HM174-100μg / 询价

BH7-HM174-500μg / 询价

BH7-HM174-500μgx2 / 询价

Human B7-H4 Protein

产品信息(Product Info)

Recombinant Human B7-H4 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Phe29-Ala258.[Accession | Q7Z7D3-1]

分子量大小(Molecular Weight)

The protein has a predicted MW of 28.2 kDa. Due to glycosylation, the protein migrates to 52-68 kDa based on Tris-Bis PAGE result.


Less than 1EU per μg by the LAL method.


> 95% as determined by Tris-Bis PAGE
> 95% as determined by HPLC


Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.


Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.


-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3-6 months after reconstitution.
2-8°C for 2-7 days after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Tris-Bis PAGE

Human B7-H4 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.


The purity of Human B7-H4 is greater than 95% as determined by SEC-HPLC.


Immobilized Human B7-H4, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-B7-H4 Antibody, hFc Tag with the EC50 of 3.1ng/ml determined by ELISA.


B7-H4, also known as B7x and B7S1, is a 50-80 kDa glycosylated member of the B7 family of immunomodulatory proteins.B7-H4 is up-regulated in several carcinomas in correlation with tumor progression and metastasis. A soluble form of B7-H4 is elevated in the serum of ovarian cancer, renal cell carcinoma, and rheumatoid arthritis patients, also in correlation with advanced disease status .


B7H4; B7-H4; B7S1; B7h.5; B7x; FLJ22418; VTCN1; PRO1291


(1)Smith J B, Caitlin S, Powell D J. B7-H4 as a potential target for immunotherapy for gynecologic cancers: a closer look[J]. Gynecologic Oncology, 2014, 134(1):181-189.

Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lypholized powder.