BST-HM101-100μg / 询价
BST-HM101-500μg / 询价
BST-HM101-500μgx2 / 询价
表达区间及表达系统(Source)
|
Recombinant Human BST1 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gly29-Ala293 [Accession | Q10588-1]. |
分子量大小(Molecular Weight)
|
The protein has a predicted MW of 31.2 kDa. Due to glycosylation, the protein migrates to 38-48 kDa based on Bis-Tris PAGE result. |
纯度(Purity)
|
> 95% as determined by Bis-Tris PAGE |
内毒素(Endotoxin)
|
Less than 1EU per μg by the LAL method. |
制剂(Formulation)
|
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
重构方法(Reconstitution)
|
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
存储(Storage)
|
-20 to -80°C for 24 months as supplied from date of receipt. |
Recombinant Human BST1 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gly29-Ala293 [Accession | Q10588-1].
The protein has a predicted MW of 31.2 kDa. Due to glycosylation, the protein migrates to 38-48 kDa based on Bis-Tris PAGE result.
> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC
Less than 1EU per μg by the LAL method.
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
-20 to -80°C for 24 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
BST1 overexpression conferred resistance to sphingosine in yeast. BST1 deletion produced sensitivity to exogenous D-erythro-sphingosine and phytosphingosine and intracellular accumulation of sphingosine 1-phosphate upon exposure to exogenous sphingosine. sphingoid base metabolism is similar in all eukaryotes and suggests that yeast genetics may be useful in the isolation and identification of other genes involved in sphingolipid signaling and metabolism.
Antigen BP3; BST-1; Bp-3; Bp3; Ly65; Bst1; BST1; CD157
(1) Saba JD, Nara F, Bielawska A, Garrett S, Hannun YA. The BST1 gene of Saccharomyces cerevisiae is the sphingosine-1-phosphate lyase. J Biol Chem. 1997 Oct 17;272(42):26087-90. doi: 10.1074/jbc.272.42.26087. PMID: 9334171.
BST-HM101-100μg / 询价
BST-HM101-500μg / 询价
BST-HM101-500μgx2 / 询价
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