BST-HM101 | 价格

BST-HM101-100μg / 询价

BST-HM101-500μg / 询价

BST-HM101-500μgx2 / 询价

Human BST1 Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Human BST1 Protein is expressed from HEK293 with His tag at the C-Terminus.
It contains Gly29-Ala293 [Accession | Q10588-1].

分子量大小(Molecular Weight)

The protein has a predicted MW of 31.2 kDa. Due to glycosylation, the protein migrates to 38-48 kDa based on Bis-Tris PAGE result.

纯度(Purity)

> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 24 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Bis-Tris PAGE

Human BST1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

ELISA Data

Immobilized Human BST1, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Anti-BST1 Antibody, hFc Tag with the EC50 of 2.5ng/ml determined by ELISA.

背景(Background)

BST1 overexpression conferred resistance to sphingosine in yeast. BST1 deletion produced sensitivity to exogenous D-erythro-sphingosine and phytosphingosine and intracellular accumulation of sphingosine 1-phosphate upon exposure to exogenous sphingosine. sphingoid base metabolism is similar in all eukaryotes and suggests that yeast genetics may be useful in the isolation and identification of other genes involved in sphingolipid signaling and metabolism.

分子别名(Synonyms)

Antigen BP3; BST-1; Bp-3; Bp3; Ly65; Bst1; BST1; CD157

文献(References)

(1) Saba JD, Nara F, Bielawska A, Garrett S, Hannun YA. The BST1 gene of Saccharomyces cerevisiae is the sphingosine-1-phosphate lyase. J Biol Chem. 1997 Oct 17;272(42):26087-90. doi: 10.1074/jbc.272.42.26087. PMID: 9334171.