货号价格

BTL-MM101-100μg / 询价

BTL-MM101-500μg / 询价

BTL-MM101-500μgx2 / 询价

Mouse BTLA Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Mouse BTLA Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Glu30-Gly176.[Accession | Q32MV9]

分子量大小(Molecular Weight)

The protein has a predicted MW of 17.8 kDa. Due to glycosylation, the protein migrates to 35-50 kDa based on Tris-Bis PAGE result.

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

纯度(Purity)

> 95% as determined by Tris-Bis PAGE
> 95% as determined by HPLC

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3-6 months after reconstitution.
2-8°C for 2-7 days after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Tris-Bis PAGE

Mouse BTLA on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Mouse BTLA is greater than 95% as determined by SEC-HPLC.

背景(Background)

B- and T-lymphocyte attenuator (BTLA; CD272) is a 35 kDa type I transmembrane glycoprotein in the CD28 family of T cell costimulatory molecules. BTLA is a inhibitory receptor on lymphocytes that negatively regulates antigen receptor signaling via PTPN6/SHP-1 and PTPN11/SHP-2. BTLA may interact in cis (on the same cell) or in trans (on other cells) with TNFRSF14.

分子别名(Synonyms)

CD272; BTLA; BTLA1; FLJ16065; MGC129743

文献(References)

(1)Haymaker C L , Wu R C , Ritthipichai K , et al. BTLA marks a less-differentiated tumor-infiltrating lymphocyte subset in melanoma with enhanced survival properties[J]. OncoImmunology, 2015, 4(8):e1014246.

Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lypholized powder.