CAS-HM112 | 价格

CAS-HM112-100μg / 询价

CAS-HM112-500μg / 询价

CAS-HM112-500μgx2 / 询价

Human CA12/Carbonic anhydrase XII Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Human CA12/Carbonic anhydrase XII Protein is expressed from HEK293 with His tag at the C-Terminus.
It contains Ala25-Ser301 [Accession | O43570-1].

分子量大小(Molecular Weight)

The protein has a predicted MW of 32.22 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Bis-Tris PAGE result.

纯度(Purity)

> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Bis-Tris PAGE

Human CA12 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Human CA12 is greater than 95% as determined by SEC-HPLC.

Bioactivity Data

Measured by its esterase activity. The specific activity is > 40 pmol/min/µg.

背景(Background)

Carbonic anhydrases (CAs) are a family of enzymes involved in the pH regulation of metabolically active cells/tissues. Carbonic anhydrase XII (CA XII) is a key mediator of several signaling pathways that are involved in cancer development.

分子别名(Synonyms)

Carbonic anhydrase; CA12; CAXII; Carbonic anhydrase 12; CA-XII; Tumor antigen HOM-RCC-3.1.3

文献(References)

(1) Williams KJ, Gieling RG. Preclinical Evaluation of Ureidosulfamate Carbonic Anhydrase IX/XII Inhibitors in the Treatment of Cancers. Int J Mol Sci. 2019 Dec 2;20(23):6080. doi: 10.3390/ijms20236080. PMID: 31810330; PMCID: PMC6928609.

(2) Gu XF, Shi CB, Zhao W. Prognostic value of carbonic anhydrase XII (CA XII) overexpression in hepatocellular carcinoma. Int J Clin Exp Pathol. 2019 Jun 1;12(6):2173-2183. PMID: 31934040; PMCID: PMC6949632.

Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.