CD1-HM217 | 价格

CD1-HM217-100μg / 询价

CD1-HM217-500μg / 询价

CD1-HM217-500μgx2 / 询价

Human CD117 Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Human CD117 Protein is expressed from HEK293 with hFc tag at the C-Terminus.
It contains Gln26-Pro520 [Accession | P10721-2].

分子量大小(Molecular Weight)

The protein has a predicted MW of 82.46 kDa. Due to glycosylation, the protein migrates to 100-130 kDa based on Bis-Tris PAGE result.

纯度(Purity)

> 95% as determined by Bis-Tris PAGE

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in 20mM PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Bis-Tris PAGE

Human CD117 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

ELISA Data

Immobilized Human SCF at 2μg/ml (100μl/well) on the plate. Dose response curve for Human CD117, hFc Tag with the EC50 of 11.3ng/ml determined by ELISA (QC Test).

ELISA Data

Immobilized Human CD117, hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti-CD117 Antibody, hFc Tag with the EC50 of 22.7ng/ml determined by ELISA.

背景(Background)

The c-kit proto-oncogen (CD 117) has been shown to be present in several cell types including normal and neoplastic hemopoietic cells. Among normal BM cells, CD117 expression has been found in about half of the CD34 precursors including progenitors committed to the erythroid, granulo-monocytic, and megakaryocytic cell lineages.

分子别名(Synonyms)

SCFR; PBT; CD117; ckit; c-kit; EC 2.7.10; EC 2.7.10.1; KIT

文献(References)

(1) Escribano L, Ocqueteau M, Almeida J, Orfao A, San Miguel JF. Expression of the c-kit (CD117) molecule in normal and malignant hematopoiesis. Leuk Lymphoma. 1998 Aug;30(5-6):459-66. doi: 10.3109/10428199809057558. PMID: 9711908.