Human CD40/TNFRSF5 Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Human CD40/TNFRSF5 Protein is expressed from HEK293 with hFc tag at the C-Terminus.
It contains Glu21-Arg193 [Accession | P25942-1].

分子量大小(Molecular Weight)

The protein has a predicted MW of 45.9 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Bis-Tris PAGE result.

纯度(Purity)

> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 24 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Bis-Tris PAGE

Human CD40 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Human CD40 is greater than 95% as determined by SEC-HPLC.

ELISA Data

Immobilized Human CD40 Ligand Trimer, His Tag at 5μg/ml (100μl/Well) on the plate. Dose response curve for Human CD40, hFc Tag with the EC50 of 101.8ng/ml determined by ELISA.

背景(Background)

CD40 is a costimulatory protein found on antigen presenting cells and is required for their activation. The binding of CD154 (CD40L) on TH cells to CD40 activates antigen presenting cells and induces a variety of downstream effects.CD40 molecule is a potential target for cancer immunotherapy. There are number of completed and ongoing clinical trials where agonistic anti-CD40 monoclonal antibodies are employed to activate an anti-tumor T cell response via activation of dendritic cells.

分子别名(Synonyms)

CD40; CD40L receptor;  TNFRSF5; CD40 antigen; CD40 molecule; CDw40; MGC9013;

文献(References)

(1)Pound, J. D. Minimal cross-linking and epitope requirements for CD40-dependent suppression of apoptosis contrast with those for promotion of the cell cycle and homotypic adhesions in human B cells[J]. International Immunology, 1999, 11(1):11-20

Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.