货号价格

CD4-HM401-100μg / 询价

CD4-HM401-500μg / 询价

CD4-HM401-1mg / 询价

Human CD4/LEU3 Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Human CD4/LEU3 Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal. It contains Lys26-Trp390.[Accession | P01730]

分子量大小(Molecular Weight)

The protein has a predicted MW of 43.6 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Tris-Bis PAGE result.

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

纯度(Purity)

> 95% as determined by Tris-Bis PAGE
> 95% as determined by HPLC

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-20 to -80°C for 3-6 months in unopened state after reconstitution.
2-8°C for 2-7 days after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please avoid freeze-thaw cycles.

产品数据(Assay Data)
Tris-Bis PAGE

Human CD4 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Human CD4 is greater than 95% as determined by SEC-HPLC

ELISA Data

Immobilized Human CD4 at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-CD4 Antibody, hFc Tag with the EC50 10.6ng/ml determined by ELISA.

Batch to Batch Consistency

Immobilized Human CD4 at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-CD4 Antibody, hFc Tag with the EC50 of 10.2ng/ml / 10.6ng/ml / 10.6ng/ml determined by ELISA.

背景(Background)

CD4, also known as L3T4, T4, and W3/25, is an approximately 55 kDa type I transmembrane glycoprotein that is expressed predominantly on thymocytes and a subset of mature T lymphocytes. It is a standard phenotype marker for the identification of T cell populations.Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class II molecule:peptide complex.

分子别名(Synonyms)

CD4 antigen (p55); CD4 molecule; CD4 receptor; CD4; CD4mut; EU3

文献(References)

(1)Lam S , Bollard C. T-cell therapies for HIV[J]. Immunotherapy, 2013, 5(4):407-414.

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