CD4-HM401B | 价格

CD4-HM401B-100μg / 询价

CD4-HM401B-500μg / 询价

CD4-HM401B-500μgx2 / 询价

Biotinylated Human CD4/LEU3 Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Biotinylated Human CD4/LEU3 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.
It contains Lys26-Trp390 [Accession | P01730].

分子量大小(Molecular Weight)

The protein has a predicted MW of 43.6 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Bis-Tris PAGE result.

纯度(Purity)

> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 24 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Bis-Tris PAGE

Biotinylated Human CD4 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Biotinylated Human CD4 is greater than 95% as determined by SEC-HPLC.

ELISA Data

Immobilized Anti-CD4 Antibody, hFc Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human CD4, His Tag with the EC50 of 8.4ng/ml determined by ELISA.

背景(Background)

CD4, also known as L3T4, T4, and W3/25, is an approximately 55 kDa type I transmembrane glycoprotein that is expressed predominantly on thymocytes and a subset of mature T lymphocytes. It is a standard phenotype marker for the identification of T cell populations.Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class II molecule:peptide complex.

分子别名(Synonyms)

CD4 antigen (p55); CD4 molecule; CD4 receptor; CD4; CD4mut; EU3

文献(References)

(1)Lam S , Bollard C. T-cell therapies for HIV[J]. Immunotherapy, 2013, 5(4):407-414.

Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.