CD7-CM147-100μg / 询价
CD7-CM147-500μg / 询价
CD7-CM147-500μgx2 / 询价
Cynomolgus/Rhesus macaque CD47 Protein
Recombinant Cynomolgus/Rhesus macaque CD47 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Gln19-Glu141.[Accession | F7A802]
The protein has a predicted MW of 15 kDa. Due to glycosylation, the protein migrates to 45-65 kDa based on Tris-Bis PAGE result.
Less than 1EU per μg by the LAL method.
> 95% as determined by Tris-Bis PAGE
> 95% as determined by HPLC
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3-6 months after reconstitution.
2-8°C for 2-7 days after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Cynomolgus/Rhesus macaque CD47 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
The purity of Cynomolgus/Rhesus macaque CD47 is greater than 95% as determined by SEC-HPLC.
Immobilized Cynomolgus/Rhesus macaque CD47, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human SIRP alpha, hFc Tag with the EC50 of 0.52ug/ml determined by ELISA (QC Test).
Cynomolgus SIRP alpha, His Tag immobilized on CM5 Chip can bind Cynomolgus /Rhesus macaque CD47, His Tag with an affinity constant of 63.27 nM as determined in SPR assay (Biacore T200).
CD47 (Cluster of Differentiation 47) also known as integrin associated protein (IAP) is a transmembrane protein that in humans is encoded by the CD47 gene. CD47 belongs to the immunoglobulin superfamily and partners with membrane integrins and also binds the ligands thrombospondin-1 (TSP-1) and signal-regulatory protein alpha (SIRPα).CD-47 acts as a don't eat me signal to macrophages of the immune system which has made it a potential therapeutic target in some cancers.
CD47 glycoprotein; CD47 molecule; CD47; IAP; OA3; MER6
(1) Weiskopf K , Ring A M , Ho C C M , et al. Engineered SIRP? Variants as Immunotherapeutic Adjuvants to Anticancer Antibodies[J]. Science, 2013, 341(6141):88-91.
