CD8-HM428-100μg / 询价
CD8-HM428-500μg / 询价
CD8-HM428-500μgx2 / 询价
Human/Cynomolgus/Rhesus macaque CD28 Protein
Recombinant Human/Cynomolgus/Rhesus macaque CD28 Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus.It contains Asn19-Pro152 [Accession | P10747-1].
The protein has a predicted MW of 18.2 kDa. Due to glycosylation, the protein migrates to 36-52 kDa based on Bis-Tris PAGE result.
>95% as determined by Bis-Tris PAGE
Less than 1 EU per μg by the LAL method.
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Human/Cynomolgus/Rhesus macaque CD28 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Immobilized Human/Cynomolgus/Rhesus macaque CD28, His Tag at 2 μg/ml (100 μl/Well) on the plate. Dose response curve for Anti-CD28 Antibody, hFc Tag with the EC50 of 23.9 ng/ml determined by ELISA (QC Test).
Human B7-1, hFc Tag captured on CM5 Chip via Protein A can bind Human/Cynomolgus/Rhesus macaque CD28, His-Avi Tag with an affinity constant of 43.61 μM as determined in SPR assay (Biacore T200).
Anti-CD28 antibody captured on Protein A chip, can bind Human/Cynomolgus/Rhesus macaque CD28, His Tag with an affinity constant of 5.15 nM as determined in a SPR assay (Biacore T200).
CD28 is the receptor for CD80 (B7-1) and CD86 (B7-2) proteins. When activated by Toll-like receptor ligands, the CD80 expression is upregulated in antigen presenting cells (APCs). The CD86 expression on antigen presenting cells is constitutive. CD28 is the only B7 receptor constitutively expressed on naive T cells.
CD28 antigen (Tp44); CD28 molecule; CD28; MGC138290
(1) Suntharalingam G , Perry M R , Ward S , et al. Cytokine Storm in a Phase 1 Trial of the Anti-CD28 Monoclonal Antibody TGN1412[J]. New England Journal of Medicine, 2006, 355(10):1018-1028.
