货号价格

CD8-HM428-100μg / 询价

CD8-HM428-500μg / 询价

CD8-HM428-1mg / 询价

Human/Cynomolgus/Rhesus macaque CD28 Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Human/Cynomolgus/Rhesus macaque CD28 Protein is expressed from Expi293 with His tag and Avi tag at the N-terminal. It contains Asn19-Pro152.[Accession | P10747-1]

分子量大小(Molecular Weight)

The protein has a predicted MW of 18.2 kDa. Due to glycosylation, the protein migrates to 36-52 kDa based on Tris-Bis PAGE result.

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

纯度(Purity)

> 95% as determined by Tris-Bis PAGE
> 95% as determined by HPLC

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-20 to -80°C for 3-6 months in unopened state after reconstitution.
2-8°C for 2-7 days after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please avoid freeze-thaw cycles.

产品数据(Assay Data)
Tris-Bis PAGE

Human/Cynomolgus/Rhesus macaque CD28 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Human/Cynomolgus/Rhesus macaque CD28 was greater than 95% as determined by SEC-HPLC.

ELISA Data

Immobilized Human/Cynomolgus/Rhesus macaque CD28, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-CD28 Antibody, hFc Tag with the EC50 of 15.7ng/ml determined by ELISA.

SPR Data

Anti-CD28 antibody captured on Protein A chip, can bind Human/Cynomolgus/Rhesus macaque CD28, His Tag with an affinity constant of 5.15nM as determined in a SPR assay (Biacore T200).

背景(Background)

CD28 is the receptor for CD80 (B7-1) and CD86 (B7-2) proteins.When activated by Toll-like receptor ligands, the CD80 expression is upregulated in antigen presenting cells (APCs). The CD86 expression on antigen presenting cells is constitutive. CD28 is the only B7 receptor constitutively expressed on naive T cells.

分子别名(Synonyms)

CD28 antigen (Tp44); CD28 molecule; CD28; MGC138290

文献(References)

(1)Suntharalingam G , Perry M R , Ward S , et al. Cytokine Storm in a Phase 1 Trial of the Anti-CD28 Monoclonal Antibody TGN1412[J]. New England Journal of Medicine, 2006, 355(10):1018-1028.

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