Biotinylated Human Claudin 18.2 Protein-Nanodisc
Recombinant Biotinylated Human Claudin 18.2 Protein-Nanodisc is expressed from HEK293 with His tag at the N-terminus and Avi tag at the C-terminus.It contains Met1-Val261 [Accession | P56856-2].
The protein has a predicted MW of 39.90 kDa.
Less than 1EU per μg by the LAL method.
Supplied as 0.22 μm filtered solution in PBS (pH 7.4). Notice: Not recommended for immunization.
Valid for 12 months from date of receipt when stored at -80°C.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Biotinylated Human Claudin 18.2 Nanodisc, His Tag at 5μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-Claudin 18.2 Antibody, hFc Tag with the EC50 of 82.2ng/ml determined by ELISA (QC Test).
Biotinylated Human Claudin 18.2 Nanodisc captured on CM5 Chip via Streptavidin can bind Anti-Claudin18.2 Antibody with an affinity constant of 7.28 nM as determined in SPR assay (Biacore T200).
Claudin18 (CLDN18) belongs to the large claudin family of proteins, which form tight junction strands in epithelial cells. CLDN18 is specifically expressed in the stomach and lung. CLDN18 has two alternatively spliced variants, CLDN18.1 and CLDN18.2. Isoform 2 (Claudin 18.2) is abundant in gastric tumors.
CLDN18; CLDN18.2; Claudin-18.2; Claudin18.2; Claudin-18; DKFZp564B2062; SFTA5; SFTPJ
(1) Singh P , Toom S , Huang Y. Anti-claudin 18.2 antibody as new targeted therapy for advanced gastric cancer[J]. Journal of Hematology & Oncology, 2017, 10(1):105.
