COV-VE0CL-100μg / 询价
COV-VE0CL-500μg / 询价
COV-VE0CL-500μgx2 / 询价
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SARS-CoV-2 3CLpro/3C-like Protease Protein
Recombinant SARS-CoV-2 3CLpro/3C-like Protease Protein is expressed from E.coli without tag. It contains Ser1-Gln306.[Accession | YP_009725301.1]
The protein has a predicted MW of 33.8 kDa same as Tris-Bis PAGE result.
Less than 1EU per μg by the LAL method.
> 95% as determined by Tris-Bis PAGE
> 95% as determined by HPLC
Supplied as 0.22μm filtered solution in 25mM HEPES, 2.5mM DTT, 10% Glycerol (pH 7.5).
Valid for 12 months from date of receipt when stored at -80°C.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
SARS-CoV-2 3CLpro on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
The purity of SARS-CoV-2 3CLpro is greater than 95% as determined by SEC-HPLC.
Measured by its ability to cleave a peptide substrate, MCA-AVLQSGFR-Lys(Dnp)-Lys-NH2.The specific activity is > 5 pmol/min/μg.
3CL protease, a viral cysteine proteinase, plays an important role in co-translational proteolytic processing of Coronavirus polyproteins. The 3CL protease cleaves as much as 11 sites in the replicase polyproteins and also releases the key replicative functions of polymerase and helicase.
3C-like protease; 3CL Protease; 3CL-Mpro; 3CL Pro; COVID-19; M Proteinase; Mpro
(1)Pillaiyar T, Manickam M, Namasivayam V, Hayashi Y, Jung SH. An Overview of Severe Acute Respiratory Syndrome-Coronavirus (SARS-CoV) 3CL Protease Inhibitors: Peptidomimetics and Small Molecule Chemotherapy. J Med Chem. 2016 Jul 28;59(14):6595-628. doi: 10.1021/acs.jmedchem.5b01461. Epub 2016 Feb 29. PMID: 26878082; PMCID: PMC7075650.
