货号价格

COV-VE1PL-100μg / 询价

COV-VE1PL-500μg / 询价

COV-VE1PL-500μgx2 / 询价

SARS-COV-2 PLpro/papain-like protease Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant SARS-COV-2 PLpro/papain-like protease Protein is expressed from E.coli with His tag at the N-Terminus. It contains Glu1564-Val1880.[Accession | YP_009725299.1]

分子量大小(Molecular Weight)

The protein has a predicted MW of 39.2 kDa same as Tris-Bis PAGE result.

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

纯度(Purity)

> 95% as determined by Tris-Bis PAGE

制剂(Formulation)

Supplied as 0.22μm filtered solution in 20mM Tris, 100mM NaCl, 10% Glycerol (pH 8.5).

存储(Storage)

Valid for 12 months from date of receipt when stored at -80°C.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Tris-Bis PAGE

SARS-COV-2 PLpro on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

背景(Background)

The coronaviral proteases, papain-like protease (PLpro) and 3C-like protease (3CLpro), are attractive antiviral drug targets because they are essential for coronaviral replication. Although the primary function of PLpro and 3CLpro are to process the viral polyprotein in a coordinated manner, PLpro has the additional function of stripping ubiquitin and ISG15 from host-cell proteins to aid coronaviruses in their evasion of the host innate immune responses.

分子别名(Synonyms)

Replicase polyprotein 1a; pp1a; nsp1; PL2-PRO; PL-PRO; Papain-like Protease; Plpro

文献(References)

(1) Báez-Santos YM, St John SE, Mesecar AD. The SARS-coronavirus papain-like protease: structure, function and inhibition by designed antiviral compounds. Antiviral Res. 2015 Mar;115:21-38. doi: 10.1016/j.antiviral.2014.12.015. Epub 2014 Dec 29. PMID: 25554382; PMCID: PMC5896749.