COV-VM1RM-100μg / 询价
COV-VM1RM-500μg / 询价
COV-VM1RM-500μgx2 / 询价
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SARS-COV-2 Spike RBD (N501Y, K417N, E484K) Protein
Recombinant SARS-COV-2 Spike RBD (N501Y,K417N,E484K) Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Arg319-Phe541(N501Y,K417N,E484K) [Accession | QHD43416.1].
The protein has a predicted MW of 26.2 kDa. Due to glycosylation, the protein migrates to 30-40 kDa based on Bis-Tris PAGE result.
> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC
Less than 1 EU per μg by the LAL method.
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
SARS-COV-2 Spike RBD (N501Y,K417N,E484K) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of SARS-COV-2 Spike RBD (N501Y,K417N,E484K) is greater than 95% as determined by SEC-HPLC.
Immobilized SARS-COV-2 Spike RBD (N501Y,K417N,E484K) , His Tag at 0.5μg/ml (100ul/Well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 9.9ng/ml determined by ELISA. (QC Test)
Loaded Human ACE2, hFc Tag on ProA-Biosensor can bind SARS-COV-2 Spike RBD (N501Y, K417N, E484K), His Tag with an affinity constant of 12.90 nM as determined in BLI assay (Gator® Prime).
The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
S protein RBD; S glycoprotein RBD; Spike protein RBD
(1)Larry J Anderson, Ralph S Baric. Emerging Human Coronaviruses - Disease Potential and Preparedness[J]. N Engl J Med, 2012, 367(19):1850-1852.
