COV-VM1SM-100μg / 询价
COV-VM1SM-500μg / 询价
COV-VM1SM-500μgx2 / 询价
SARS-COV-2 Spike S1 (N501Y,K417N,E484K) Protein
Recombinant SARS-COV-2 Spike S1 (N501Y,K417N,E484K) Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Val16-Arg685(N501Y,K417N,E484K).[Accession | YP_009724390.1]
The protein has a predicted MW of 75.9 kDa. Due to glycosylation, the protein migrates to 90-130 kDa based on Tris-Bis PAGE result.
Less than 1EU per μg by the LAL method.
> 95% as determined by Tris-Bis PAGE
> 95% as determined by HPLC
Supplied as 0.22μm filtered solution in PBS (pH 7.4).
Valid for 12 months from date of receipt when stored at -80°C.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
SARS-COV-2 Spike S1 (N501Y,K417N,E484K) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
The purity of SARS-COV-2 Spike S1 (N501Y,K417N,E484K) is greater than 95% as determined by SEC-HPLC.
Immobilized SARS-COV-2 Spike S1 (N501Y,K417N,E484K) , His Tag at 0.5μg/ml (100ul/Well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 0.02μg/ml determined by ELISA.
The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
S1 protein; Spike protein S1; Spike,S1 protein; S glycoprotein Subunit1
(1)Belouzard S , Chu V C , Whittaker G R. Activation Of The Sars Coronavirus Spike Protein Via Sequential Proteolytic Cleavage At Two Distinct Sites[J]. Proceedings of the National Academy of Sciences of the United States of America, 2009, 106(14):5871-5876.
