COV-VM4LG-100μg / 询价
COV-VM4LG-500μg / 询价
COV-VM4LG-500μgx2 / 询价
SARS-COV-2 Spike S Trimer (D614G) Protein
Recombinant SARS-COV-2 Spike S Trimer(D614G) Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Val16-Glu1188 (D614G).[Accession | QHD43416.1]
The protein has a predicted MW of 136.7 kDa. Due to glycosylation, the protein migrates to 175-230 kDa based on Tris-Bis PAGE result.
Less than 1EU per μg by the LAL method.
> 95% as determined by Tris-Bis PAGE
> 95% as determined by HPLC
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3-6 months after reconstitution.
2-8°C for 2-7 days after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
SARS-COV-2 Spike S Trimer (D614G) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
The purity of SARS-COV-2 Spike S Trimer (D614G) is greater than 95% as determined by SEC-HPLC.
Immobilized SARS-COV-2 Spike S Trimer (D614G), His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 14.0ng/ml determined by ELISA.
The SARS-CoV-2 spike (S) protein variant D614G supplanted the ancestral virus worldwide, reaching near fixation in a matter of months. Recently, that D614G was been found more infectious than the ancestral form on human lung cells, colon cells, and on cells rendered permissive by ectopic expression of human ACE2 or of ACE2 orthologs from various mammals, including Chinese rufous horseshoe bat and Malayan pangolin.
S protein; Spike glycoprotein; S glycoprotein; COVID-19
(1)Yurkovetskiy L, Wang X, Pascal KE, Tomkins-Tinch C, Nyalile TP, Wang Y, Baum A, Diehl WE, Dauphin A, Carbone C, Veinotte K, Egri SB, Schaffner SF, Lemieux JE, Munro JB, Rafique A, Barve A, Sabeti PC, Kyratsous CA, Dudkina NV, Shen K, Luban J. Structural and Functional Analysis of the D614G SARS-CoV-2 Spike Protein Variant. Cell. 2020 Oct 29;183(3):739-751.e8. doi: 10.1016/j.cell.2020.09.032. Epub 2020 Sep 15. PMID: 32991842; PMCID: PMC7492024.
