Biotinylated SARS-COV-2 Spike S1 Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Biotinylated SARS-COV-2 Spike S1 Protein is expressed from HEK293 with hFc tag and Avi tag at the C-Terminus. It contains Gln14-Arg683.[Accession | QHD43416.1]

分子量大小(Molecular Weight)

The protein has a predicted MW of 102.6 kDa. Due to glycosylation, the protein migrates to 130-140 kDa based on Tris-Bis PAGE result.

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

纯度(Purity)

> 95% as determined by Tris-Bis PAGE
> 95% as determined by HPLC

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3-6 months after reconstitution.
2-8°C for 2-7 days after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Tris-Bis PAGE

Biotinylated SARS-COV-2 Spike S1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Biotinylated SARS-COV-2 Spike S1 is greater than 95% as determined by SEC-HPLC.

ELISA Data

Immobilized Biotinylated SARS-COV-2 Spike S1, hFc Tag at 2μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Human ACE2, His Tag with the EC50 of 0.09μg/ml determined by ELISA.

背景(Background)

The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

分子别名(Synonyms)

S1 protein; Spike protein S1; Spike,S1 protein; S glycoprotein Subunit1

文献(References)

(1)Belouzard S , Chu V C , Whittaker G R. Activation Of The Sars Coronavirus Spike Protein Via Sequential Proteolytic Cleavage At Two Distinct Sites[J]. Proceedings of the National Academy of Sciences of the United States of America, 2009, 106(14):5871-5876.

Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lypholized powder.