Human DNAM-1/CD226 Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Human DNAM-1/CD226 Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal. It contains Glu19-Asn247.[Accession | Q15762]

分子量大小(Molecular Weight)

The protein has a predicted MW of 28.9 kDa. Due to glycosylation, the protein migrates to 45-70 kDa based on Tris-Bis PAGE result.

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

纯度(Purity)

> 95% as determined by Tris-Bis PAGE
> 95% as determined by SEC-HPLC

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-20 to -80°C for 3-6 months in unopened state after reconstitution.
2-8°C for 2-7 days after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please avoid freeze-thaw cycles.

产品数据(Assay Data)
Tris-Bis PAGE

Human DNAM-1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Human DNAM-1 is greater than 95% as determined by SEC-HPLC.

SPR Data

Human CD155, hFc Tag captured on Protein A chip, can bind Human DNAM-1, His Tag with an affinity constant of 0.22μM as determined in a SPR assay (Biacore T200).

背景(Background)

DNAX accessory molecule-1 (DNAM-1), also known as CD226, is a 65 kDa type I transmembrane glycoprotein in the immunoglobulin superfamily.DNAM-1 mediates cellular adhesion to other cells bearing its ligands, CD112 and CD155, and cross-linking DNAM-1 with antibodies causes cellular activation. Furthermore, DNAM-1 can interact with PVR and PVRL2.

分子别名(Synonyms)

CD226 molecule; CD226; DNAM1; PTA1; TLiSA1

文献(References)

(1)Yu X , Harden K , C Gonzalez L , et al. The surface protein TIGIT suppresses T cell activation by promoting the generation of mature immunoregulatory dendritic cells[J]. NATURE IMMUNOLOGY, 2008, 10(1):48-57.

恺佧生物科技(上海)有限公司
电话:400-614-0008
地址:上海市浦江高科技园新骏环路188号8A区四楼
在线留言