DKK-HM51C-100μg / 询价
DKK-HM51C-500μg / 询价
DKK-HM51C-500μgx2 / 询价
Human DKK1 C terminal Domain Protein
Recombinant Human DKK1 C terminal Domain Protein is expressed from HEK293 with hFc tag and Avi tag at the C-Terminus.It contains Met178-His266 [Accession | O94907].
The protein has a predicted MW of 38.69 kDa. Due to glycosylation, the protein migrates to 45-55 kDa based on Bis-Tris PAGE result.
>95% as determined by Bis-Tris PAGE
Less than 1 EU per μg by the LAL method.
Lyophilized from 0.22 μm filtered solution in 20mM NaAc,150mM NaCl (pH 5.0). Normally 8% trehalose is added as protectant before lyophilization.
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in 20mM NaAc,150mM NaCl (pH 5.0).
-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Human DKK1 C terminal Domain on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Immobilized Human DKK1 C terminal Domain, hFc Tag at 0.5 μg/ml (100 μl/Well) on the plate. Dose response curve for Biotinylated Anti-DKK1 Antibody, hFc Tag with the EC50 of 26.7 ng/ml determined by ELISA.
Dickkopf-1 (Dkk1), the founding and best-studied member of the Dkk family, functions as an antagonist of canonical Wnt/β-catenin. Dkk1 is considered to play a broad role in a variety of biological processes.
Dickkopf-related 1; Dickkopf-1; Dkk-1; Dkk1; hDkk-1; hDkk1; SK
Liang L, Tu Y, Lu J, Wang P, Guo Z, Wang Q, Guo K, Lan R, Li H, Liu P. Dkk1 exacerbates doxorubicin-induced cardiotoxicity by inhibiting the Wnt/β-catenin signaling pathway. J Cell Sci. 2019 May 16;132(10):jcs228478. doi: 10.1242/jcs.228478. PMID: 31028181.
