DR3-MM101-100μg / 询价
DR3-MM101-500μg / 询价
DR3-MM101-500μgx2 / 询价
Mouse DR3/TNFRSF25 Protein
Recombinant Mouse DR3/TNFRSF25 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Gln31-Met196 [Accession | B1AWN9].
The protein has a predicted MW of 18.79 kDa. Due to glycosylation, the protein migrates to 38-48 kDa based on Bis-Tris PAGE result.
> 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC
Less than 1 EU per μg by the LAL method.
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
2-8°C for 2-7 days after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Mouse DR3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Mouse DR3 is greater than 95% as determined by SEC-HPLC.
Immobilized Mouse DR3, His Tag at 1μg/ml (100 μl/well) on the plate. Dose response curve for Biotinylated Mouse TNFSF15, His Tag with the EC50 of 8.8 ng/ml determined by ELISA (QC Test).
Mouse TNFSF15, hFc Tag captured on CM5 Chip via Protein A can bind Mouse DR3, His Tag with an affinity constant of 4.56 nM as determined in SPR assay (Biacore T200).
Death Receptor 3 (DR3), also known as TNFRSF25, TRAMP, LARD, or WSL-1, is a death-domain-containing TNF-family receptor that, like its closest paralog TNFR1, binds the adaptor molecule TRADD through its cytoplasmic death domain. TRADD recruitment endows DR3 with dual-signaling capability to activate NF−κB and MAP-kinase signaling or alternatively trigger caspase activation and programmed cell death.
APO3; DDR3; DR3; TNFRSF12; WSL; WSL1; TNFRSF25; LARD; TR3; TRAMP; WSL-LR
Meylan F, Davidson TS, Kahle E, Kinder M, Acharya K, Jankovic D, Bundoc V, Hodges M, Shevach EM, Keane-Myers A, Wang EC, Siegel RM. The TNF-family receptor DR3 is essential for diverse T cell-mediated inflammatory diseases. Immunity. 2008 Jul 18;29(1):79-89. doi: 10.1016/j.immuni.2008.04.021. Epub 2008 Jun 19. PMID: 18571443; PMCID: PMC2760084.
