EPH-MM110 | 价格

EPH-MM110-100μg / 询价

EPH-MM110-500μg / 询价

EPH-MM110-500μgx2 / 询价

Mouse EPHA10 Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Mouse EPHA10 Protein is expressed from HEK293 with His tag at the C-Terminus.
It contains Leu23-Ala565 [Accession | Q8BYG9-1].

分子量大小(Molecular Weight)

The protein has a predicted MW of 59.6 kDa. Due to glycosylation, the protein migrates to 68-80 kDa based on Bis-Tris PAGE result.

纯度(Purity)

> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Bis-Tris PAGE

Mouse EPHA10 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Mouse EPHA10 is greater than 95% as determined by SEC-HPLC.

背景(Background)

EphA10 (erythropoietin-producing hepatocellular carcinoma receptor A10) is a catalytically defective receptor protein tyrosine kinase in the ephrin receptor family.EphA10 was elevated and higher in tumor tissues than in normal tissues in some cancer types, including pancreatic cancer.EphA10 silencing reduced the proliferation, migration, and adhesion of MIA PaCa-2 and AsPC-1 pancreatic cancer cells. EphA10 plays a pivotal role in the tumorigenesis of pancreatic epithelial cells and is a novel therapeutic target for pancreatic cancer.

分子别名(Synonyms)

EphA10; FLJ16103; FLJ33655; MGC43817

文献(References)

(1) Shin WS, Park MK, Lee YH, Kim KW, Lee H, Lee ST. The catalytically defective receptor protein tyrosine kinase EphA10 promotes tumorigenesis in pancreatic cancer cells. Cancer Sci. 2020 Sep;111(9):3292-3302. doi: 10.1111/cas.14568. Epub 2020 Jul 27. PMID: 32644283; PMCID: PMC7469775.

Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.