FAP-HM101-100μg / 询价
FAP-HM101-500μg / 询价
FAP-HM101-500μgx2 / 询价
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Human FAP Protein
Recombinant Human FAP Protein is expressed from HEK293 with His tag at the N-Terminus.It contains Leu26-Asp760 [Accession | Q12884-1].
The protein has a predicted MW of 86.1 kDa. Due to glycosylation, the protein migrates to 90-100 kDa based on Bis-Tris PAGE result.
> 95% as determined by Bis-Tris PAGE
Less than 1 EU per μg by the LAL method.
Lyophilized from 0.22μm filtered solution in 20mM Tris,0.25M NaCl (pH 8.2). Normally 8% trehalose is added as protectant before lyophilization.
Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.
-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Human FAP on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.
Immobilized Human FAP, His Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Anti-FAP Antibody, hFc Tag with the EC50 of 12.3ng/ml determined by ELISA (QC Test).
Measured by its ability to convert the substrate benzyloxycarbonyl-Gly-Pro-7-amido-4-methylcoumarin (Z-GP-AMC) to Z-Gly-Pro and 7-amino-4-methylcoumarin (AMC). The specific activity is >3000 pmol/min/µg (QC Test).
Fibroblast activation protein (FAP) is a serine protease that has been reported in fibroblasts and some carcinoma cells, which correlates with poor patient outcomes. FAP can be induced under hypoxia which is also vital in the malignant behaviors of cancer cells.
FAP; FAPalpha; SIMP; Seprase; APCE; DPPIV; DPPIVA; FAPA
(1)Zou B, Liu X, Zhang B, et al. The Expression of FAP in Hepatocellular Carcinoma Cells is Induced by Hypoxia and Correlates with Poor Clinical Outcomes. J Cancer. 2018;9(18):3278-3286. Published 2018 Sep 7. doi:10.7150/jca.25775.
