货号价格

FCR-HM101B-100μg / 询价

FCR-HM101B-500μg / 询价

FCR-HM101B-500μgx2 / 询价

Biotinylated Human FcRH5/FcRL5 Protein (Primary Amine Labeling)

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Biotinylated Human FcRH5/FcRL5 Protein (Primary Amine Labeling) is expressed from HEK293 with His tag at the C-Terminus.
It contains Gln16-Gly851 [Accession | Q96RD9-1].

分子量大小(Molecular Weight)

The protein has a predicted MW of 92.38 kDa. Due to glycosylation, the protein migrates to 100-120 kDa based on Bis-Tris PAGE result.

纯度(Purity)

> 95% as determined by Bis-Tris PAGE
> 90% as determined by HPLC

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS, 100mM Arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Bis-Tris PAGE

Biotinylated Human FcRH5 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Biotinylated Human FcRH5 is greater than 90% as determined by SEC-HPLC.

ELISA Data

Immobilized Anti-FcRH5 Antibody, hFc Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human FcRH5, His Tag with the EC50 of 35.6ng/ml determined by ELISA (QC Test).

背景(Background)

FcRH5 is a cell surface marker enriched on malignant plasma cells when compared to other hematologic malignancies and normal tissues. DFRF4539A is an anti-FcRH5 antibody-drug conjugated to monomethyl auristatin E (MMAE), a potent anti-mitotic agent.

分子别名(Synonyms)

FcR-like protein 5; FcRH5; FcRL5; BXMAS1; BXMAS1MGC119592; CD307; CD307e; DKFZp667E2019; DKFZp667F216; FCRH5MGC119593; FLJ00333; FLJ00397; IFGP5; IRTA2

文献(References)

(1)Elkins K, Zheng B, Go M, et al. FcRL5 as a target of antibody-drug conjugates for the treatment of multiple myeloma. Mol Cancer Ther. 2012;11(10):2222-2232. doi:10.1158/1535-7163.MCT-12-0087.

Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.