FCR-MM162-100μg / 询价
FCR-MM162-500μg / 询价
FCR-MM162-500μgx2 / 询价
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Mouse Fc gamma RIV/CD16-2 Protein
Recombinant Mouse Fc gamma RIV/CD16-2 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gly21-Gln203 [Accession | A0A0B4J1G0-1].
The protein has a predicted MW of 22 kDa. Due to glycosylation, the protein migrates to 32-43 kDa based on Bis-Tris PAGE result.
> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC
Less than 1EU per μg by the LAL method.
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Mouse Fc gamma RIV on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Mouse Fc gamma RIV is greater than 95% as determined by SEC-HPLC.
Mouse Fc gamma RIV, His Tag captured on CM5 Chip via anti-his antibody can bind Anti-CD3 Antibody with an affinity constant of 146 nM as determined in SPR assay (Biacore T200).
FcgammaRIV is a relatively new IgG Fc receptor (FcgammaR) that is reported to contribute to the pathogenesis of autoimmune diseases.FcgammaRIII and FcgammaRIV are each essential to trigger an FcRgamma-linker for activation of T-cell-dependent signal that drives C5a production in the Arthus reaction. A combined requirement for FcgammaRIII and FcgammaRIV in autoimmune injury, and identify the linker for activation of T cells adaptor as an integral component of linked FcgammaR and C5a anaphylatoxin receptor activation to generate inflammation.
CD16-2; FcgammaRIV; Fcgr4; FcγRIV
(1) Syed SN, Konrad S, Wiege K, Nieswandt B, Nimmerjahn F, Schmidt RE, Gessner JE. Both FcgammaRIV and FcgammaRIII are essential receptors mediating type II and type III autoimmune responses via FcRgamma-LAT-dependent generation of C5a. Eur J Immunol. 2009 Dec;39(12):3343-56. doi: 10.1002/eji.200939884. PMID: 19795417.
