FER-HM201-100μg / 询价
FER-HM201-500μg / 询价
FER-HM201-500μgx2 / 询价
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Human Fc epsilon RI alpha/FCER1a Protein
Recombinant Human Fc epsilon RI alpha/FCER1a Protein is expressed from HEK293 with hFc tag at the C-Terminus. It contains Val26-Gln205.[Accession | P12319-1]
The protein has a predicted MW of 47.8 kDa. Due to glycosylation, the protein migrates to 70-78 kDa based on Tris-Bis PAGE result.
Less than 1EU per μg by the LAL method.
> 95% as determined by Tris-Bis PAGE
> 95% as determined by HPLC
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3-6 months after reconstitution.
2-8°C for 2-7 days after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Human Fc epsilon RI alpha on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
The purity of Human Fc epsilon RI alpha is greater than 95% as determined by SEC-HPLC.
Immobilized Human Fc epsilon RI alpha, hFc Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-Fc epsilon RI alpha Antibody, hFc Tag with the EC50 14.5ng/ml determined by ELISA.
Known susceptibility genes to atopy and asthma have been identified by linkage or associations with clinical phenotypes, including total serum IgE levels. IgE-mediated sensitivity reactions require a high-affinity IgE receptor (FcepsilonRI), which immobilizes the immunoglobulin on the surface of the effector cells, mostly mast cells and basophils. Similarly to the previously investigated beta subunit of the receptor, FCER1A is a good candidate for a quantitative trait locus (QTL) in allergic diseases, and appears to participate in the systemic regulation of IgE levels.
Fc-epsilon RI-alpha; FcERI; FCER1A; FCE1A
(1) Sanak M, Potaczek DP, Nizankowska-Mogilnicka E, Szczeklik A. Genetic variability of the high-affinity IgE receptor alpha subunit (Fc epsilon RI alpha) is related to total serum IgE levels in allergic subjects. Allergol Int. 2007 Dec;56(4):397-401. doi: 10.2332/allergolint.R-07-145. Epub 2007 Nov 1. PMID: 17965580.
