FGF-HE001 | 价格

FGF-HE001-100μg / 询价

FGF-HE001-500μg / 询价

FGF-HE001-500μgx2 / 询价

Human FGF basic Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Human FGF basic Protein is expressed from E.coli without tag
It contains Pro143-Ser288 [Accession | P09038-4].

分子量大小(Molecular Weight)

The protein has a predicted MW of 16.5 kDa same as Bis-Tris PAGE result.

纯度(Purity)

> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC

内毒素(Endotoxin)

Less than 0.02 EU per μg by the LAL method.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, 8% trehalose (pH 7.4).

重构方法(Reconstitution)

Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Bis-Tris PAGE
Human FGF basic on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
SEC-HPLC
The purity of Human FGF basic is greater than 95% as determined by SEC-HPLC.
Cell Based Assay
Measured in a cell proliferation assay using BALB/c 3T3 mouse embryonic fibroblasts. The ED50 for this effect is 0.1 - 0.6 ng/ml (QC Test).
ELISA Data
Immobilized Human FGF basic at 1μg/ml (100μl/Well) on the plate. Dose response curve for Human Glypican 1, His Tag with the EC50 of 11.1ng/ml determined by ELISA. (QC Test)
 
Immobilized Human FGF basic, No Tag at 5 μg/ml (100 μl/well) on the plate. Dose response curve for Human FGFR2 beta (IIIc), His Tag with the EC50 of 0.49 μg/ml determined by ELISA.
背景(Background)

FGF basic is a member of the FGF family of at least 23 related mitogenic proteins which show 35-60% amino acid conservation. FGF acidic and basic, unlike the other members of the family, lack signal peptides and are apparently secreted by mechanisms other than the classical protein secretion pathway.

分子别名(Synonyms)

bFGF; FGF basic; FGF2; FGF-2; FGF2AS; GFG1; HBGF-2; NUDT6; Prostatropin; BFGF; FGFB; FGFβ; HBGF2;FGF beta;Fibroblast growth factor-2; Fibroblast growth factor 2

文献(References)

(1) Zhiwu J , Xiaofeng J , Suimin C , et al. Anti-GPC3-CAR T Cells Suppress the Growth of Tumor Cells in Patient-Derived Xenografts of Hepatocellular Carcinoma[J]. Frontiers in Immunology, 2017, 7.

(2)Ho M , Kim H. Glypican-3: A new target for cancer immunotherapy[J]. European Journal of Cancer, 2011, 47(3):0-338.

Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.