货号价格

FGF-HE001-100μg / 询价

FGF-HE001-500μg / 询价

FGF-HE001-500μgx2 / 询价

Human FGF basic Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Human FGF basic Protein is expressed from E.coli without tag.
It contains Pro143-Ser288 [Accession | P09038-4].

分子量大小(Molecular Weight)

The protein has a predicted MW of 16.5 kDa same as Tris-Bis PAGE result.

内毒素(Endotoxin)

Less than 0.02 EU per μg by the LAL method.

纯度(Purity)

> 95% as determined by Tris-Bis PAGE

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3-6 months after reconstitution.
2-8°C for 2-7 days after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Tris-Bis PAGE

Human FGF basic on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

ELISA Data

Immobilized Human FGF basic at 0.5 μg/ml (100 μl/Well) on the plate. Dose response curve for Human Glypican 1, His Tag with the EC50 of 12.6 ng/ml determined by ELISA (QC Test).

ELISA Data

Immobilized Human FGF basic, No Tag at 5 μg/ml (100 μl/well) on the plate. Dose response curve for Human FGFR2 beta (IIIc), His Tag with the EC50 of 1.18 μg/ml determined by ELISA.

Cell Based Assay

Measured in a cell proliferation assay using BALB/c 3T3 mouse embryonic fibroblasts. The ED50 for this effect is 0.02 - 0.1 ng/ml (QC Test).

背景(Background)

FGF basic is a member of the FGF family of at least 23 related mitogenic proteins which show 35-60% amino acid conservation. FGF acidic and basic, unlike the other members of the family, lack signal peptides and are apparently secreted by mechanisms other than the classical protein secretion pathway.

分子别名(Synonyms)

bFGF; FGF basic; FGF2; FGF-2; FGF2AS; GFG1; HBGF-2; NUDT6; Prostatropin; BFGF; FGFB; FGFβ; HBGF2

文献(References)

(1) Zhiwu J , Xiaofeng J , Suimin C , et al. Anti-GPC3-CAR T Cells Suppress the Growth of Tumor Cells in Patient-Derived Xenografts of Hepatocellular Carcinoma[J]. Frontiers in Immunology, 2017, 7.

(2)Ho M , Kim H. Glypican-3: A new target for cancer immunotherapy[J]. European Journal of Cancer, 2011, 47(3):0-338.