Human FGF10 Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Human FGF10 Protein is expressed from E.coli without tag.
It contains Gln38-Ser208 [Accession | O15520].

分子量大小(Molecular Weight)

The protein has a predicted MW of 19.3 kDa. The protein migrates to 25 kDa based on Tris-Bis PAGE result.

内毒素(Endotoxin)

Less than 0.1EU per μg by the LAL method.

纯度(Purity)

> 90% as determined by Tris-Bis PAGE

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in 20mM Tris, 150mM NaCl (pH 8.0). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3-6 months after reconstitution.
2-8°C for 2-7 days after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Tris-Bis PAGE

Human FGF10 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

SPR Data

Human FGF10, No Tag immobilized on CM5 Chip can bind Human FGFR2 beta (IIIb) , hFc Tag with an affinity constant of 0.299 nM as determined in SPR assay (Biacore T200).

背景(Background)

Fibroblast growth factor 10 (FGF10) regulates multiple stages of structural lung morphogenesis, cellular differentiation, and the response to injury. As a driver of lung airway branching morphogenesis, FGF10 signaling defects during development lead to neonatal lung disease. Lung diseases impact patients across the lifespan, from infants in the first minutes of life through the aged population. Congenital abnormalities of lung structure can cause lung disease at birth or make adults more susceptible to chronic disease.

分子别名(Synonyms)

FGF-10; KGF2

文献(References)

(1) Prince LS. FGF10 and Human Lung Disease Across the Life Spectrum. Front Genet. 2018 Oct 31;9:517. doi: 10.3389/fgene.2018.00517. PMID: 30429870; PMCID: PMC6220039.