FGF-HE40AB | 价格

FGF-HE40AB-100μg / 询价

FGF-HE40AB-500μg / 询价

FGF-HE40AB-500μgx2 / 询价

Biotinylated Human FGF1 Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Biotinylated Human FGF1 Protein is expressed from E.coli with His tag and Avi tag at the N-terminus.
It contains Phe16-Asp155 [Accession | P05230-1].

分子量大小(Molecular Weight)

The protein has a predicted MW of 18.74 kDa. Due to glycosylation, the protein migrates to 20-24 kDa based on Tris-Bis PAGE result.

纯度(Purity)

> 95% as determined by Bis-Tris PAGE

内毒素(Endotoxin)

Less than 1 EU per μg by the LAL method.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Bis-Tris PAGE

Biotinylated Human FGF1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

ELISA Data

Immobilized Human FGFR1 alpha (IIIc), His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human FGF1, His-Avi Tag with the EC50 of 100.7ng/ml determined by ELISA (QC Test).

背景(Background)

Fibroblast Growth Factor 1 (Fgf1), also known as acidic FGF (aFGF), is involved in the regulation of various biological processes, ranging from development to disease pathogenesis. It is a single chain polypeptide and is highly expressed in adult brain and kidney tissues. Its expression has been shown to be directed by multiple tissue-specific promoters, which generate transcripts of varying lengths.

分子别名(Synonyms)

FGF-1; aFGF; ECGF; HBGF-1; FGFA

文献(References)

Jamal SB, Hockman D. FGF1. Differentiation. 2024 Sep-Oct;139:100802. doi: 10.1016/j.diff.2024.100802. Epub 2024 Jul 22. PMID: 39074995.