FGF-HM204 | 价格

FGF-HM204-100μg / 询价

FGF-HM204-500μg / 询价

FGF-HM204-500μgx2 / 询价

Human FGF4 Protein

COA MSDS
产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Human FGF4 Protein is expressed from HEK293 with hFc (IgG1) tag at the C-terminus.
It contains Ala31-Leu206 [Accession | P08620-1].

分子量大小(Molecular Weight)

The protein has a predicted MW of 45.23 kDa. Due to glycosylation, the protein migrates to 52-62 kDa based on Bis-Tris PAGE result.

纯度(Purity)

> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC

内毒素(Endotoxin)

Less than 0.1 EU per μg by the LAL method.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Dissolve the lyophilized protein in distilled water. Please refer to the Certificate of Analysis for detailed instructions.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Bis-Tris PAGE
Human FGF4 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
SEC-HPLC
The purity of Human FGF4 is greater than 95% as determined by SEC-HPLC.
ELISA Data
Immobilized Human FGFR3 alpha (IIIb), His Tag (Cat. FGF-HM43B) at 1μg/ml (100μl/well) on the plate. Dose response curve for Human FGF4, hFc Tag with the EC50 of 4.29μg/ml determined by ELISA. (QC Test)
背景(Background)

Fibroblast growth factor-4 (FGF-4), a highly mitogenic protein encoded by the k-fgf/hst oncogene, stimulates the growth of a variety of cells of mesenchymal and neuroectodermal origin. It is a member of the FGF family and plays several important roles in bone development during embryogenesis.

分子别名(Synonyms)

FGF-4; FGF 4; HST; HST-1; HSTF-1; HBGF-4; KS3;FGF4; HST1; HSTF1; HBGF4;Fibroblast growth factor-4; Fibroblast growth factor 4

文献(References)

Quito FL, Beh J, Bashayan O, Basilico C, Basch RS. Effects of fibroblast growth factor-4 (k-FGF) on long-term cultures of human bone marrow cells. Blood. 1996 Feb 15;87(4):1282-91. PMID: 8608216.

Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.