FGL-HM211 | 价格

FGL-HM211-100μg / 询价

FGL-HM211-500μg / 询价

FGL-HM211-500μgx2 / 询价

Human FGL1 Protein

产品信息(Product Info)
表达区间及表达系统(Source)

Recombinant Human FGL1 Protein is expressed from HEK293 with hFc tag at the N-Terminus.
It contains Asp64-Asn305 [Accession | Q08830].

分子量大小(Molecular Weight)

The protein has a predicted MW of 54.8 kDa. Due to glycosylation, the protein migrates to 60-66 kDa based on Bis-Tris PAGE result.

纯度(Purity)

> 95% as determined by Bis-Tris PAGE
> 90% as determined by HPLC

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in 20mM PB, 250mM NaCl (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

存储(Storage)

-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

产品数据(Assay Data)
Bis-Tris PAGE

Human FGL1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SEC-HPLC

The purity of Human LTBR is greater than 90% as determined by SEC-HPLC.

ELISA Data

Immobilized Human LAG3, His Tag at 5μg/ml (100μl/Well) on the plate. Dose response curve for Human FGL1, hFc Tag with the EC50 of 0.28μg/ml determined by ELISA.

背景(Background)

Fibrinogen-like protein 1 (FGL-1) is a protein that is structurally related to fibrinogen. In humans, FLG-1 is encoded by the FGL1 gene.Fibrinogen-like protein 1 is a member of the fibrinogen family of proteins, which also includes fibrinogen, fibrinogen-like protein 2, and clotting factors V, VIII, and XIII.Fibrinogen-like Protein 1 is a major immune inhibitory ligand of LAG-3.

分子别名(Synonyms)

FGL1; FGL-1; Hepassocin; HP-041; HFREP-1; LFIRE-1; HFREP1; Lag3 ligand

文献(References)

(1)Wang, J., Sanmamed, M.F., Datar, I., Su, T.T., Ji, L., Sun, J., Chen, L., Chen, Y., Zhu, G., Yin, W., et al. (2019). Fibrinogen-like Protein 1 Is a Major Immune Inhibitory Ligand of LAG-3. Cell 176, 334-347.e312.

Note: HPLC results always come from liquid protein, due to absorption peak interference of trehalose in lyophilized powder.